REGULATORY MUTATIONS IN THE THREONINE OPERON

苏氨酸操纵子的调控突变

• 批准号: 3275997
• 负责人: JEFFREY F GARDNER
• 金额: $ 13.13万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-12-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3275997
• 关键词: DNA; Escherichia coli; bacterial virus; bacteriophage lambda; beta galactosidase; enzyme induction /repression; frameshift mutation; gene expression; gene mutation; genetic mapping; genetic recombination; genetic strain; genetic transcription; lac operon; molecular cloning; mutant; nucleic acid sequence; operon; plasmids; radiotracer; structural genes; threonine; virus genetics

Regulatory mutants which affect attenuation of the threonine (thr) operon will be isolated and studied. Recently developed in vitro mutagenesis techniques will be employed to direct mutations into specific sites in the thr regulatory region. Mutants will be characterized both by DNA sequencing and by their regulatory effects in vivo. The positions of the mutations and their effects on regulation will supply information on the relationship between secondary RNA structure and transcription termination. Another aspect of the proposal is concerned with generating mutations in the recombination site (att site) used by bacteriophage Lambda during site-specific recombination. Mutations will be induced in vitro and characterized by their effects on recombination in vivo and by their interactions with Lambda integrase (int) protein in DNA protection and binding assays.

影响苏氨酸操纵子衰减的调节突变体 将被隔离和研究。 最近开发的体外诱变 技术将被用来指导突变到特定的位点， Thr调节区。 突变体的特征将通过DNA 测序和它们在体内的调节作用。 的位置 突变及其对监管的影响将提供有关 RNA二级结构与转录终止的关系。 该提案的另一个方面是关于产生突变， 噬菌体λ在重组过程中使用的重组位点（att位点） 位点特异性重组。 将在体外诱导突变， 其特征在于它们对体内重组的作用以及它们的 与λ整合酶（int）蛋白在DNA保护中的相互作用， 结合测定。