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BACTERIAL CELL SURFACE STRUCTURE FUNCTIONS & BIOGENESIS

细菌细胞表面结构功能

基本信息

批准号：
2459330
负责人：
PAUL D RICK
金额：
$ 16.77万
依托单位：
HENRY M. JACKSON FDN FOR THE ADV MIL/MED
依托单位国家：
美国
项目类别：
财政年份：
1980
资助国家：
美国
起止时间：
1980-08-01 至 1998-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2459330
关键词：
Escherichia coli Salmonella typhimurium acylation apolipoproteins bacterial proteins copper ion transport ligase metal metabolism nonblood lipoprotein peptidoglycan protein biosynthesis protein purification protein structure function

项目摘要

DESCRIPTION: (adapted from investigator's abstract) A rapidly increasing number of membrane proteins in bacteria have been found to contain an N- terminal lipoamino acid, N-acyl-diacylglycerylcysteine, the hallmark of all bacterial lipoproteins. As of 1993, more than 130 lipoproteins have been identified in both gram positive and gram negative bacteria covering a wide spectrum of genera and species. The biosynthesis of lipoproteins in bacteria is both temporally and spatially related to the export of these membrane proteins across the cytoplasmic membranes. These proteins are made as precursor proteins with N-terminal signal sequences which contain a "lipobox" sequence at the junction of the signal sequences and the mature proteins. Following the interaction of the precursor proteins with the export machinery defined by the SecA, SecY SecD and SecF proteins, the prolipoproteins are modified and processed by three enzymes located in the cytoplasmic membrane. These three enzymes are prolipoprotein diacylglyceral transferase, prolipoprotein signal peptidase and apolipoprotein N- acyltransferase encoded by the lgt, lsp, and lnt genes, respectively, in Escherichia coli and Salmonella typhimurium. Mutants defective in any of these three genes are conditionally lethal. In addition, lnt mutants are also copper sensitive, and the lnt gene is allelic with the cutE gene, one of six genes postulated to be involved in copper transport and homeostasis in E. coli. Using the E. coli major outer membrane lipoprotein as the model system, the investigator proposes; (i) to elucidate the biochemical basis for the essential nature of the lipoprotein biosynthetic pathway in E. coli or S. typhimurium; (ii) to identify the gene encoding lipoprotein:peptidoglycan ligase in E. coli; (iii) to study the structure, function and assembly of the prolipoprotein modification and processing enzymes; and (iv) to study the interrelationship between apolipoprotein N-acylation and the transport and homeostasis of divalent cations such as Cu++ in E. coli. The results of the proposed studies will provide new information regarding the structures, functions and biogenesis of lipoproteins in bacteria. These studies will also provide a rational basis for the search or design of antibacterial agents which utilize the enzymes of this pathway as targets.

描述：（改编自研究者的摘要）A迅速