CHARGE TRANSFER ACROSS BIOLOGICAL INTERFACES

跨生物界面的电荷转移

• 批准号: 2402884
• 负责人: DAVID C MAUZERALL
• 金额: $ 17.53万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-07-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2402884
• 关键词: bacteriorhodopsins; cell morphology; charge transfer complex; chloride ion; electron transport; ionic bond; lipid bilayer membrane; microorganism culture; neurons; protein structure function; rhodopsin; spectrometry; thermodynamics; voltage gated channel

DESCRIPTION: The basic goal of the work is to understand the factors affecting interfacial and transmembrane charge transfer in lipid bilayers. The work is potentially important, since the maintenance of ion gradients across membranes is one of the defining qualities of living systems. The approach characteristic of this work is the use of photoinduced transfer of electrons across lipid bilayers. This permits the study of internal electrostatic effects. In one phase of the work, it is proposed to apply this method to voltage gated ion channels, as an effort to gain photo-control over channel activity. The extension of the method to nerve cells is a possible direction of the research. A second avenue involves the further study and development of a device for electrostatic ion pumping that was created in Dr. Mauzerall's laboratory. Again, the ions are driven across the membrane by photoinduced charge transfer across an interface. This device allows the accurate calibration of electrostatic effects in transmembrane phenomena. The third project involves the determination of parameters of the biological ion pumps bacteriorhodopsin which pumps protons, and halorhodopsin which pumps chloride ions. The enthalpy and volume changes are associated with various steps undergone by these photoinduced pumps. These experiments will involve photoacoustic spectroscopy, a specialty of Dr. Mauzerall.

描述：这项工作的基本目标是了解影响因素 影响脂质双层中的界面和跨膜电荷转移。 这项工作可能很重要，因为离子梯度的维持 跨膜是生命系统的决定性品质之一。 这 这项工作的方法特点是使用光诱导转移 电子穿过脂质双层。 这使得研究内部 静电效应。 在工作的一个阶段，建议将此方法应用于电压 门控离子通道，作为获得通道光控制的努力 活动。 该方法有可能扩展到神经细胞 研究方向。 第二条途径涉及进一步研究和 开发静电离子泵装置 莫泽拉尔博士的实验室。 再次，离子被驱动穿过膜 通过界面上的光致电荷转移。 该设备允许 跨膜现象中静电效应的精确校准。 第三个项目涉及生物参数的确定。 离子泵细菌视紫红质（泵送质子）和盐菌视紫红质（泵送质子） 泵送氯离子。 焓和体积的变化与 这些光诱导泵经历的各个步骤。 这些实验将 涉及光声光谱学，这是 Mauzerall 博士的专长。