CHARGE TRANSFER ACROSS INTERFACES OF BIOLOGICAL SYSTEMS

本研究旨在了解电荷转移的机制 跨越生物界面。 该有限深度的界面区域是 在生物过程中的重要性， 细胞间的相互作用。 选择性光引发电荷的动力学 局部化在界面处的转移反应被用作这种情况的探针。 脂质双层的区域。 动力学的电场依赖性 带电的疏水颜料将被用来研究性质极性 和介电梯度）。 刚性给体-受体复合物 将合成在双层-水界面处特异性定向的 并用于在短（ns）时间尺度上探测界面。 质子 在该区域中的转移之后将是合适的施主或受主， 例如醌类。 荷电的和未荷电的两种离子的跨膜渡越时间均为 不带电荷的物种将通过这些光引发的单界面测量 电荷转移反应和合适的氧化还原、pH或离子梯度。 的 使用快速电压和电流放大器进行测量， 快速数字化 光源为闪光灯和N_2激光泵浦染料 激光

This research is aimed at understanding the mechanism of charge transfer across biological interfaces. This interfacial region of finite depth is of importance in biological processes which range from energy transduction to cell-cell interactions. The kinetics of selected photoinitiated charge transfer reactions localized at the interface are used as probes of this region of the lipid bilayer. The electric field dependence of the kinetics of charged hydrophobic pigments will be used to study properties polarity and dielectric gradients) of this region. Rigid donor-acceptor complexes specifically orientable at the bilayer-water interface will be synthesized and used to probe the interface on the short (ns) time scale. Proton transfers in this region will be followed by suitable donors or acceptors, e.g. quinones. The cross membrane transit time of both charged and uncharged species will be measured by these photoinitiated single interface charge transfer reactions and suitable redox, pH or ion gradients. The measurements are made with fast voltage and current amplifiers and very fast digitizers. The light sources are flash lamp and N2 laser pumped dye lasers.