CHARGE TRANSFER ACROSS INTERFACES OF BIOLOGICAL SYSTEMS

生物系统界面间的电荷转移

• 批准号: 3273256
• 负责人: DAVID C MAUZERALL
• 金额: $ 13.35万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-07-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3273256
• 关键词: charge transfer complex; chemical reaction; electrochemistry; electron transport; ion transport; lasers; mathematics; membrane model; membrane permeability; membrane potentials; photochemistry; porphyrins; quinones

This research is aimed at understanding the mechanism of charge transfer across biological interfaces. This interfacial region of finite depth is of importance in biological processes which range from energy transduction to cell-cell interactions. The kinetics of selected photoinitiated charge transfer reactions localized at the interface are used as probes of this region of the lipid bilayer. The electric field dependence of the kinetics of charged hydrophobic pigments will be used to study properties polarity and dielectric gradients) of this region. Rigid donor-acceptor complexes specifically orientable at the bilayer-water interface will be synthesized and used to probe the interface on the short (ns) time scale. Proton transfers in this region will be followed by suitable donors or acceptors, e.g. quinones. The cross membrane transit time of both charged and uncharged species will be measured by these photoinitiated single interface charge transfer reactions and suitable redox, pH or ion gradients. The measurements are made with fast voltage and current amplifiers and very fast digitizers. The light sources are flash lamp and N2 laser pumped dye lasers.

这项研究旨在了解电荷转移的机理。 跨越生物界面。这个有限深度的界面区域是 在生物过程中的重要性，从能量转导到 细胞与细胞之间的相互作用。选择性光引发电荷的动力学 界面上的转移反应被用作这方面的探针。 脂双层的区域。动力学的电场依赖性 将用于研究带电疏水颜料的性质和极性 和介电梯度)。刚性给体-受体络合物 可在双层-水界面上特别定向的将被合成 并用于在短(Ns)时间尺度上探测界面。质子 在这一地区的转移之后将有合适的捐赠者或接受者， 例如，对苯二酚。带电膜和带电膜的跨膜渡越时间 未带电物种将通过这些光引发的单一界面来测量 电荷转移反应和适当的氧化还原、pH或离子梯度。这个 使用快速电压和电流放大器进行测量， 快速数字化仪。光源为闪光灯和氮气激光泵浦染料 激光。