CEREBELLUM AND NM CONDITIONING IN ANESTHETIZED RABBITS

本申请中提出的研究的长期目标是 描述神经元系统和过程参与简单形式的 学习和记忆。更具体地说，这项研究旨在促进我们的 理解小脑参与简单运动 学习任务，经典眼睑条件反射。三个实验 使用麻醉的经典条件反射制剂。 这些准备工作涉及到大脑微刺激的配对到位 和记录来自大脑的单位活动 已知参与产生条件反射的干核， 监控明显的行为反应的地方。拟议的实验 包括：（1）小脑细胞外单个单位活动分析 在条件反射期间和之后，（2）对“行为”的操纵 在麻醉条件反射期间使用的训练方案，以及（3） 调节后细胞内记录与HRP标记偶联。 这些实验旨在探索相似性， 清醒和麻醉家兔之间的条件反射差异， 提供小脑神经元活动变化的信息 诱导的麻醉条件反射程序，并提供一些 深入了解小脑和脑干神经元之间的连接 参与了条件反射。这种麻醉剂的进一步发展 条件准备应有助于定义和研究 小脑和脑干回路在这种形式的学习过程中被激活。 神经元活动的细胞内分析应该提供初步的 有关细胞过程和突触机制的信息 经典的眼睑调节。我们预计这些机制 可以推广到其他运动学习和记忆情况， 从而进一步了解小脑和脑干的基本功能 神经元功能这些实验的结果也应该证明是有用的 以了解与小脑相关的正常功能丧失 由损伤或疾病引起的病理。此外，提高我们的知识 参与简单运动学习的细胞和突触过程， 记忆应该被证明对理解更复杂的学习有用， 记忆过程以及学习障碍和记忆的细胞基础 与疾病和伤害有关的损失。

The long-term goal of research proposed in the present application is to delineate neuronal systems and processes involved in simple forms of learning and memory. More specifically, this research seeks to advance our understanding of the involvement of the cerebellum in a simple motor learning task, classical eyelid conditioning in rabbits. Three experiments are proposed that use anesthetized classical conditioning preparations. These preparations involve the pairing of brain microstimulation in place of peripheral conditioning stimuli and recording unit activity from brain stem nuclei known to be involved in generating conditioning responses in place of monitoring overt behavioral responses. The proposed experiments include: (1) An analysis of extracellular single unit cerebellar activity during and after conditioning, (2) Manipulations of the "behavioral" training regimen used during the anesthetized conditioning, and (3) Intracellular recordings coupled with HRP labeling after conditioning. These experiments have been designed to explore similarities and differences in conditioning between awake and anesthetized rabbits, to provide information about changes in the activity of cerebellar neurons induced by the anesthetized conditioning procedures, and to provide some insight into the connectivity between cerebellar and brain stem neurons involved in the conditioning. The further development of this anesthetized conditioning preparation should facilitate the definition and study of cerebellar and brain stem circuits activated during this form of learning. Intracellular analyses of neuronal activity should provide initial information about cellular processes and synaptic mechanisms associated with classical eyelid conditioning. We anticipate that these mechanisms may be generalizable to other motor learning and memory situations and therefore advance our understanding of basic cerebellar and brainstem neuronal function. Results from these experiments should also prove useful for understanding loss of normal function associated with cerebellar pathologies caused by injury or disease. Moreover, advancing our knowledge of cellular and synaptic processes involved in simple motor learning and memory should prove useful for understanding more complex learning and memory processes and the cellular bases of learning impairments and memory loss associated with disease and injury.