IMAGE READY FIBER OPTIC SENSORS FOR BIOMEDICAL ANALYSIS

用于生物医学分析的图像就绪光纤传感器

• 批准号: 2412203
• 负责人: DAVID R. WALT
• 金额: $ 25.4万
• 依托单位: TUFTS UNIVERSITY MEDFORD
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2412203
• 关键词: acetylcholine; analytical method; biomedical equipment development; charge coupled device camera; chemical structure; dyes; fiber optics; fluorescence; histochemistry /cytochemistry; imaging /visualization /scanning; monitoring device; nucleic acid sequence; oligonucleotides; stainings; tissue /cell culture

DESCRIPTION (Adapted from applicant's abstract): The work described in this application is aimed at preparing optical sensors using imaging fibers for biomedical analysis. Imaging fibers are high density coherent optical arrays with thousands of individual micron-sized optical fibers drawn into a coherent bundle with a total diameter of 200-500 microns. Two approaches are taken and lead to sensors with very different uses. In the first approach, the imaging sensor is modified with a multitude of fluorescent indicating regions on the distal face. The fluorescent signal from each region is spatially resolved and can be detected with a CCD camera. In this way, multiple analytes can be monitored simultaneously using a single sensing substrate with extremely small dimensions. The planned work extends the capabilities of such sensors to genetic analysis. A novel approach is proposed in which sensors are prepared using bead libraries and in which the location of each sensing region is randomized. Using advanced pattern recognition schemes coupled with encoding, the sensors should be readily calibrated. The approach offers the potential to create high density sensor arrays capable of monitoring hundreds of analytes or gene sequences simultaneously. In addition, the extremely small overall size of the sensor enables sensitive measurements to be made on small (nanoliter) sample sizes. In the second approach, the array is coated with a thin polymer layer containing a single sensing material. The resulting sensor is a microarray containing thousands of 2-4 micron sensors. This microarray can be used to simultaneously view a biological specimen and measure chemical concentrations at the surface of the specimen. The work proposes to explore the ability of such microsensor arrays to view neuronal tissue and determine localized release of acetylcholine from individual cells in intact tissue. The ultimate goal of this work is to develop techniques for obtaining simultaneous morphological and chemical information using a minimally-invasive sensor. Such sensors could be used for in vivo biopsies, cell function and diagnosis as well as to address a wide variety of fundamental biomedical research problems.

描述（改编自申请人的摘要）：本文中描述的工作 该应用旨在使用成像光纤制备光学传感器 生物医学分析。 成像光纤是高密度相干光纤 具有数千个单独的微米级光纤的阵列，这些光纤被拉成一个 总直径为200-500微米的相干束。 两种方法 被采用并导致传感器具有非常不同的用途。 在第一个 方法，成像传感器被修改为大量荧光 指示远端面上的区域。 每个的荧光信号 该区域具有空间分辨率，可以使用 CCD 相机进行检测。 在这个 方式，可以使用单个同时监测多种分析物 尺寸极小的传感基板。 计划工作延长 此类传感器进行遗传分析的能力。 一种新颖的方法是 提出其中使用珠库制备传感器并且其中 每个传感区域的位置是随机的。 使用高级模式 识别方案与编码相结合，传感器应该很容易 已校准。 该方法提供了创建高密度传感器的潜力 能够监测数百种分析物或基因序列的阵列 同时地。 此外，传感器的整体尺寸极小 能够对小（纳升）样品进行灵敏测量。 在第二种方法中，阵列涂有薄聚合物层 包含单一传感材料。 由此产生的传感器是一个微阵列 包含数千个 2-4 微米传感器。 该微阵列可用于 同时查看生物样本并测量化学物质 样品表面的浓度。 该工作建议探索 这种微传感器阵列观察神经元组织并确定的能力 完整组织中单个细胞局部释放乙酰胆碱。 这项工作的最终目标是开发获得的技术 使用同步形态和化学信息 微创传感器。 这种传感器可用于体内活组织检查， 细胞功能和诊断以及解决各种问题 基本的生物医学研究问题。