GENETIC AND BIOCHEMICAL BASES FOR S MUTANS VIRULENCE

变异链球菌毒力的遗传和生化基础

• 批准号: 2458583
• 负责人: ROY CURTISS III
• 金额: $ 23.94万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2458583
• 关键词: Escherichia coli k12; Streptococcus mutans; antibacterial antibody; bacterial DNA; bacterial antigens; bacterial genetics; carbohydrate metabolism; carbohydrate transport; cell membrane; cell wall; dental plaque; enzyme structure; genetic manipulation; genetic recombination; genetic regulation; glycogen; host organism interaction; laboratory rabbit; messenger RNA; microorganism immunology; microorganism metabolism; molecular biology; molecular cloning; nucleic acid sequence; oral bacteria; oxidoreductase; phosphotransferases; protein transport; pyrimidines; recombinant DNA; transferase; virulence

Genetic, biochemical and immunological approaches will be used to investigate the genetic and biochemical bases for the virulence of the Streptococcus mutans group S. mutans, S. sobrinus, S. cricetus, and S. rattus of cariogenic bacteria. S. mutans DNA has been and will continue to be cloned into appropriate Escherichia coli K-12 hosts. Clones specifying information thought to be important in contributing to the ability of S. mutans to colonize and display virulence will be characterized. Methods will be developed using cloned gene probes to quantify mRNA levels to investigate regulation of S. mutans genes for colonization and virulence attributes and for enzymes of intermediary carbohydrate metabolism. The specific projects to be pursued are to: (i) continue molecular genetic analysis of the spaA gene and biochemical characterization of the SpaA protein in relation to its functions and interactions with other surface macromolecules, (ii) continue molecular genetic analysis of the dex gene and to characterize dextranase and its endogenous inhibitor to define their roles in adherence and in glucan synthesis, (iii) continue modification of the asd gene encoding beta-aspartate semialdehyde dehydrogenase for use in cloning vectors and to establish mechanism(s) for regulation of the asd gene in relation to pyrimidine synthesis and cell wall assembly, (iv) clone genes for the phosphotransferase system (PTS) and conduct studies to establish mechanisms for sugar transport and utilization, and (v) clone genes for glycogen synthesis and breakdown and conduct studies to determine how these genes are regulated. These studies will contribute to understanding how S. mutans maintains metabolic activity, including ability for acid production, in the plaque environment with variation in the availability of nutrients and in response to activities of other plaque microorganisms. These studies should also provide information on how S. mutans protects itself when exposed to environmental stresses, especially those that interfere with cell wall or cell membrane synthesis or function. The research will be done in conformance with the NIH guidelines for recombinant DNA research.

将采用遗传、生化和免疫学方法

项目成果

Overproduction of a dextranase inhibitor by Streptococcus sobrinus mutants.

远缘链球菌突变体过量产生葡聚糖酶抑制剂。

• DOI: 10.1128/jb.176.23.7206-7212.1994
• 发表时间: 1994
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Wanda,SY;Camilli,A;Murchison,HM;Curtiss3rd,R
• 通讯作者: Curtiss3rd,R

Purification, characterization, and specificity of dextranase inhibitor (Dei) expressed from Streptococcus sobrinus UAB108 gene cloned in Escherichia coli.

大肠杆菌中克隆的远缘链球菌 UAB108 基因表达的葡聚糖酶抑制剂 (Dei) 的纯化、表征和特异性。

• DOI: 10.1128/jb.177.7.1703-1711.1995
• 发表时间: 1995
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Sun,JW;Wanda,SY;Curtiss3rd,R
• 通讯作者: Curtiss3rd,R

Purification and characterization of Streptococcus sobrinus dextranase produced in recombinant Escherichia coli and sequence analysis of the dextranase gene.

重组大肠杆菌中产生的远缘链球菌葡聚糖酶的纯化和表征以及葡聚糖酶基因的序列分析。

• DOI: 10.1128/jb.176.13.3839-3850.1994
• 发表时间: 1994
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Wanda,SY;Curtiss3rd,R
• 通讯作者: Curtiss3rd,R

Cross-reactivity between the immunodominant determinant of the antigen I component of Streptococcus sobrinus SpaA protein and surface antigens from other members of the Streptococcus mutans group.

远缘链球菌 SpaA 蛋白的抗原 I 成分的免疫显性决定簇与来自变形链球菌群其他成员的表面抗原之间的交叉反应性。

• DOI: 10.1128/iai.58.7.2276-2282.1990
• 发表时间: 1990
• 期刊: Infection and immunity
• 影响因子: 3.1
• 作者: Goldschmidt,RM;Curtiss3rd,R
• 通讯作者: Curtiss3rd,R

Cloning and DNA sequencing of the dextranase inhibitor gene (dei) from Streptococcus sobrinus.

远缘链球菌葡聚糖酶抑制剂基因 (dei) 的克隆和 DNA 测序。

• DOI: 10.1128/jb.176.23.7213-7222.1994
• 发表时间: 1994
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Sun,JW;Wanda,SY;Camilli,A;Curtiss3rd,R
• 通讯作者: Curtiss3rd,R