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IDENTIFICATION OF NIDDM GENES IN THE PIMA INDIAN TRIBE

皮马印第安部落 NIDDM 基因的鉴定

基本信息

批准号：
2518354
负责人：
DONALD W BOWDEN
金额：
$ 27.3万
依托单位：
WAKE FOREST UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1993
资助国家：
美国
起止时间：
1993-09-30 至 1999-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2518354
关键词：
Native Americans diabetes mellitus genetics family genetics genetic markers genetic polymorphism glucose tolerance test human genetic material tag insulin sensitivity /resistance linkage mapping molecular cloning noninsulin dependent diabetes mellitus obesity

项目摘要

The genetics of non-insulin dependent diabetes (NIDDM; Type 2 diabetes) will be studied in the Pima Indian Tribe. This collaborative study will include the efforts of molecular geneticists, epidemiologists and clinicians focused on finding the inherited factors which lead to NIDDM. A large number of multiplex families and sib pairs have been identified and collected and some DNA typing and analysis has been carried out already. Initially efforts will be focused on 4 regions of human chromosomes which show some evidence for linkage to NIDDM using sib-pair analysis. In addition, numerous candidate genes will also be surveyed and a systematic survey of the genome will be continued using highly polymorphic microsatellite repeat polymorphisms and restriction fragment length polymorphisms. Data will be analyzed using a number of different linkage analysis approaches including sib-pair analysis, conventional linkage analysis, and also quantitative measures of traits such as obesity, glucose tolerance and insulin resistance. Detection of linkage will be followed by detailed molecular genetic analysis to determine the basis of NIDDM susceptibility. Linkages will also be tested in other populations and ethnic groups.

非胰岛素依赖型糖尿病（NIDDM）的遗传学将在皮马印第安部落进行研究这项合作研究将包括分子遗传学家、流行病学家和临床医生集中精力寻找导致NIDDM的遗传因素。大量的多重家系和同胞对已被确定并进行了DNA分型和分析已经. 最初的努力将集中在人类的4个区域显示出与NIDDM连锁的证据的染色体分析. 此外，还将调查众多的候选基因并将继续使用高度的基因组进行系统性调查多态性微卫星重复序列多态性和限制性片段长度多态性将使用多种不同的连锁分析方法，包括同胞对分析，传统的连锁分析，以及性状的定量测量，肥胖、葡萄糖耐量和胰岛素抵抗。连锁检测随后将进行详细的分子遗传分析，以确定 NIDDM易感性的基础。还将在其他国家测试联系人口和种族群体。