EFFECT OF LOW PROTEIN INTAKE ON THE SYNTHESIS OF PLASMA PROTEINS

低蛋白质摄入量对血浆蛋白合成的影响

• 批准号: 6247041
• 负责人: NAOMI K FUKAGAWA
• 金额: $ 2.44万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-23 至 1997-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6247041
• 关键词: albumins; apolipoproteins; blood proteins; clinical research; dietary proteins; gas chromatography mass spectrometry; glucose tolerance; human subject; insulin sensitivity /resistance; nutrition related tag; protein biosynthesis; very low density lipoprotein

The major aim of this study was to determine whether insulin resistance/glucose intolerance is related to the alterations in the fractional synthesis rates of albumin and apolipoprotein B. The approach used was the primed, constant infusion of L-{I,2-13C2} leucine with gas chromatography/mass spectrometry analysis of the 13C enrichment in hydrolyzed samples of apo B and albumin. Eight volunteers were studied between 12/1/95 and 5/7/96. Laboratory analyses are pending. Technical difficulties with VLDL isolation and mass spectrometry sensitivity in the albumin enrichments were encountered.

本研究的主要目的是确定胰岛素是否 抵抗/葡萄糖不耐受与 白蛋白和载脂蛋白 B 的分数合成率。 使用的是用气体引发的、持续输注的 L-{I,2-13C2} 亮氨酸 13C富集的色谱/质谱分析 apo B 和白蛋白的水解样品。研究了八名志愿者 95 年 12 月 1 日至 96 年 5 月 7 日之间。实验室分析正在进行中。技术的 VLDL 分离和质谱灵敏度的困难 遇到白蛋白富集。