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TEMPLATE DIRECTED COVALENT CROSS LINKING OF DNA

TEMPLATE DIRECTED COVALENT CROSS LINKING OF DNA
DNA 模板定向共价交联
批准号:
2634703
负责人:
ROBERT S COLEMAN
金额:
$10.91万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1995
资助国家:
美国
项目状态:
已结题
起止时间:
1995-01-01 至 1999-12-31

项目摘要

项目成果

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中文摘要
翻译
探针直接作用对基因表达的抑制作用
英文摘要
Inhibition of genetic expression by the direct interaction of probe molecules with specifically targeted sequences of DNA or mRNA is a fundamental approach to the chemotherapeutic treatment of viral and oncogenic diseases. Selective inhibition of gene expression using antisense oligonucleotides exploits the base-pairing capability of DNA or mRNA to escort the antisense probe to a targeted sequence of nucleic acids, thereby making this approach to chemotherapy exceptionally specific. The studies described in this proposal use a "modified nucleic acid" strategy for template-directed covalent alkylation of DNA. This process will be achieved by the chemical synthesis of suitably functionalized modified nucleic acids, the incorporation of these bases into oligonucleotides, and the post-synthetic attachment of an electrophilic tether. Upon duplex formation with a complementary oligonucleotide strand, a covalent cross-link will be formed between the probe oligonucleotide and a nucleophilic group on the complementary strand. The consequence of this process will be the creation of an indelible lesion that will irreversibly mask the genetic information contained within the targeted oligonucleotide molecule. Such covalent oligonucleotide probes could provide the basis for a valuable new class of highly specific pharmacological agents for antisense inhibition of oncogenic, genetic, and viral diseases.
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SAXS STUDIES ON ENDOGENEOUS HUMAN TFID
  • 批准号:
    7370518
  • 项目类别:
  • 资助金额:
    $0.24万
  • 财政年份:
    2006
  • 负责人:
    ROBERT S COLEMAN
  • 依托单位:
Asymmetric Synthesis of Cytotoxic Natural Products
  • 批准号:
    6742115
  • 项目类别:
  • 资助金额:
    $2.87万
  • 财政年份:
    2002
  • 负责人:
    ROBERT S COLEMAN
  • 依托单位:
Asymmetric Synthesis of Cytotoxic Natural Products
  • 批准号:
    6624122
  • 项目类别:
  • 资助金额:
    $26.26万
  • 财政年份:
    2002
  • 负责人:
    ROBERT S COLEMAN
  • 依托单位:
Asymmetric Synthesis of Cytotoxic Natural Products
  • 批准号:
    6882619
  • 项目类别:
  • 资助金额:
    $26.26万
  • 财政年份:
    2002
  • 负责人:
    ROBERT S COLEMAN
  • 依托单位:
海外基金