COMPLETE GENOME SEQUENCE OF MYCOBACTERIUM TUBERCULOSIS

描述(改编自调查者摘要)：本报告的目标 该项目是为了确定人类染色体的完整DNA序列 结核分枝杆菌，结核病的病原体。大约一点 据信，世界上三分之一的人口患有潜伏的结核病。 尽管对结核分枝杆菌的生物学知之甚少，但结核病 人们普遍认为，从1950年的S到1980年的S， 通过使用多种药物治疗和更好的生活条件。 然而，自20世纪80年代中期以来，S的收入出现了惊人的增长。 发达国家和发展中国家的结核病感染率 这主要归因于艾滋病毒流行的扩大，衰败 公共卫生服务，以及多重耐药的出现 肺结核。今天，结核病仍然是主要的死亡原因，原因是 全球范围内的感染。很明显，完整的基因组DNA序列和 一组重组克隆将为 结核分枝杆菌研究。根据最近的演示，完整的 可以从微生物中快速获得基因组序列，并且成本低廉 实际上，结核分枝杆菌基因组计划现在是可行的。 该方法将是一种全基因组随机测序策略。这将是 通过构建随机小插入质粒库来实现 结核分枝杆菌H37Rv株的核苷酸序列分析 35,000个克隆(70,000个序列片段)。的结尾的顺序。 一组可用的最小重叠的COSMID克隆将提供 脚手架结构将最大限度地减少与GAP相关的工作 填充并提供对底层组装结构的确认。这个 组装的基因组将通过识别各种不同的 结构特征以及对开放的基因和功能角色的分配 基于数据库相似性搜索的读框。这一方法将 加快了解结核分枝杆菌生物学和意志的研究 影响，例如疫苗开发，毒力基因的识别， 认识多重耐药机制，合理用药 心理治疗。这项研究得出的数据将存放在McyDB中 这将使研究人员能够访问关于 序列、功能、克隆和其他物理地图功能 产生并与其他特征相关联，如抗原、抗体、基因位点 和Medline参考文献。

DESCRIPTION (Adapted from the Investigator's Abstract): The goal of this project is to determine the complete DNA sequence of the chromosome of Mycobacterium tuberculosis, the causative agent in tuberculosis. About one third of the world's population is believed to harbor latent tuberculosis. Despite little knowledge about the biology of M. tuberculosis, tuberculosis was widely thought to be under control from the 1950's through the 1980's through the use of multiple drug therapy and better living conditions. However, since the mid 1980's there has been an alarming increase in the rate of tuberculosis infection in developed as well as in developing nations that is primarily attributable to the expanding HIV epidemic, decaying public health services, and the emergence of multiple-drug resistant tuberculosis. Today, tuberculosis remains the leading cause of death due to infection worldwide. It is clear that the complete genomic DNA sequence and a set of recombinant clones would provide a tremendous resource for the study of M. tuberculosis. With the recent demonstration that the complete genome sequence can be obtained from microbial organisms rapidly and cost effectively, the M. tuberculosis genome project is now feasible. The approach will be a whole genome random sequencing strategy. This will be accomplished by constructing a random small insert plasmid library from M. tuberculosis strain H37Rv and sequencing the ends from approximately 35,000 clones (70,000 sequence fragments). The sequencing of the ends of a set of available minimally overlapping cosmid clones will provide a scaffolding structure that will minimize the effort associated with gap filling and provide confirmation of the underlying assembled structure. The assembled genome will then be annotated by identifying a variety of structural features as well as assigning genes and functional roles to open reading frames based on database similarity searches. This approach will accelerate studies in understanding the biology of M. tuberculosis and will impact for example vaccine development, identification of virulence genes, understanding mechanisms of multiple drug resistance, and rational drug therapy. The data developed from this study will be deposited in McyDB which will allow researchers to access the large amount of information on sequences, functions, clones, and other physical map features that will be generated and linked to other features such as antigen, antibody, gene locus and MedLine references.