COMPLETE GENOME SEQUENCE OF MYCOBACTERIUM TUBERCULOSIS

描述（改编自研究者摘要）：本研究的目标 项目是确定染色体的完整DNA序列， 结核分枝杆菌，结核病的病原体。 约一 世界上三分之一的人口被认为患有潜伏性肺结核。 尽管对M.肺结核 从20世纪50年代到80年代， 通过使用多种药物治疗和更好的生活条件。 然而，自20世纪80年代中期以来， 发达国家和发展中国家的结核病感染率 这主要是由于艾滋病毒流行的扩大， 公共卫生服务，以及出现多重耐药 结核 今天，结核病仍然是导致死亡的主要原因， 感染全球。 很明显，完整的基因组DNA序列和 一组重组克隆将为人类提供巨大的资源， 研究M.结核 随着最近的证明， 基因组序列可以从微生物中快速获得， 有效地，M.结核病基因组计划现在是可行的。 该方法将是全基因组随机测序策略。 这将 通过构建随机小插入质粒文库来完成， M.结核菌株H37Rv，并对大约 35，000个克隆（70，000个序列片段）。 一个序列的末端的排序 一组可用的最小重叠粘粒克隆将提供 脚手架结构，将尽量减少与间隙相关的努力 填充并提供下面的组装结构的确认。 的 组装的基因组然后将通过鉴定多种 结构特征以及分配基因和功能角色以打开 基于数据库相似性搜索的阅读帧。 这种方法将 加快对M.肺结核与意志 影响例如疫苗开发、毒力基因鉴定 了解多药耐药机制，合理用药 疗法 从这项研究中开发的数据将存放在McyDB中 这将使研究人员能够访问大量的信息， 序列、功能、克隆和其他物理地图功能， 产生并与其他特征如抗原、抗体、基因座 和MedLine参考文献。

DESCRIPTION (Adapted from the Investigator's Abstract): The goal of this project is to determine the complete DNA sequence of the chromosome of Mycobacterium tuberculosis, the causative agent in tuberculosis. About one third of the world's population is believed to harbor latent tuberculosis. Despite little knowledge about the biology of M. tuberculosis, tuberculosis was widely thought to be under control from the 1950's through the 1980's through the use of multiple drug therapy and better living conditions. However, since the mid 1980's there has been an alarming increase in the rate of tuberculosis infection in developed as well as in developing nations that is primarily attributable to the expanding HIV epidemic, decaying public health services, and the emergence of multiple-drug resistant tuberculosis. Today, tuberculosis remains the leading cause of death due to infection worldwide. It is clear that the complete genomic DNA sequence and a set of recombinant clones would provide a tremendous resource for the study of M. tuberculosis. With the recent demonstration that the complete genome sequence can be obtained from microbial organisms rapidly and cost effectively, the M. tuberculosis genome project is now feasible. The approach will be a whole genome random sequencing strategy. This will be accomplished by constructing a random small insert plasmid library from M. tuberculosis strain H37Rv and sequencing the ends from approximately 35,000 clones (70,000 sequence fragments). The sequencing of the ends of a set of available minimally overlapping cosmid clones will provide a scaffolding structure that will minimize the effort associated with gap filling and provide confirmation of the underlying assembled structure. The assembled genome will then be annotated by identifying a variety of structural features as well as assigning genes and functional roles to open reading frames based on database similarity searches. This approach will accelerate studies in understanding the biology of M. tuberculosis and will impact for example vaccine development, identification of virulence genes, understanding mechanisms of multiple drug resistance, and rational drug therapy. The data developed from this study will be deposited in McyDB which will allow researchers to access the large amount of information on sequences, functions, clones, and other physical map features that will be generated and linked to other features such as antigen, antibody, gene locus and MedLine references.