FUNCTION OF HORIZONTAL BASAL CELLS IN REGENERATION

嗅觉上皮的独特之处在于它能够替代濒死的神经元。 在一生中。基底细胞负责这种再生。 容量和可分为两组，水平基底细胞(HbCs) 和基于球糖的细胞(GBCS)。GBCS被认为是直接的 神经元前体不断地取代在 每天正常暴露在环境中。这些细胞也会对 直接损伤上皮，切除其靶器官，以及 通过增加嗅神经的增殖率和 神经元的产生。触发这种增加所涉及的信号 神经元变性后的增殖尚不清楚。重债穷国作为 一种检测神经元变性的中间信号细胞 和再生的启动。此外，人血清白蛋白具有表皮生长。 在再生过程中表现为活跃的因子(EGF)受体。这个 建议的实验旨在检查表皮生长因子与 HbCs受体活性与神经元发育过程 退化/再生。为了解决这个问题，手术切除了 将进行嗅觉轴突以启动退化和再生。 嗅觉感受器神经元。免疫细胞化学与嗅觉 上皮细胞外植体培养也将被用于检测这一过程。 了解再生所涉及的机制是长期的。 对逆转神经系统损伤的影响。

The olfactory epithelium is unique in its ability to replace dying neurons throughout life. The basal cells are responsible for this regenerative capacity and can be divided in two groups, horizontal basal cells (HBCs) and globose based cells (GBCs). The GBCs are considered the immediate neuronal precursors that continually replace neurons that degenerate during normal daily exposure to the environment. These cells also respond to direct lesions of the epithelium, removal of their target organ, and olfactory nerve transection by increasing their rate of proliferation and production of neurons. The signals involved in triggering this increase in proliferation after neuronal degeneration are unknown. The HBCs serve as an intermediate signaling cell in the detection of neuronal degeneration and initiation of regeneration. In addition, HBCs possess epidermal growth factor (EGF) receptors that appear to the active during regeneration. The proposed experiments are designed to examine the relationship between EGF receptor activity of HBCs and the process of neuronal degeneration/regeneration. To address this issue, surgical transection of olfactory axons will be performed to initiate degeneration and regeneration of olfactory receptor neurons. Immunocytochemistry and olfactory epithelial explant cultures will also be utilized to examine this process. Understanding the mechanisms involved in regeneration have long term implications for reversing lesions to the nervous system.