MAMMALIAN CELLS EXPRESSING FMO

兔FM01、FM02和人FM03的cDNA将在AHH-1TK/-中表达 人淋巴母细胞、AS52中国仓鼠细胞和C3H10T1/2小鼠 细胞。FMO的表达将由区域和 立体选择性/酶分析和免疫印迹。水平和稳定性 将在表达FMO的细胞系中监测FMO的表达。这个 人类FM03 Will等位基因变异对异体代谢的影响 接受检查。表达FMO的人淋巴母细胞系将 对一组表达细胞色素P450的现有细胞系的赞誉 酵素。AS52细胞将可以方便地分析突变谱 由FMO激活的前诱变剂。C3H10T1/2细胞系将允许 体外毒理学研究向恶性转化的延伸 终结点。 建议的商业应用：哺乳动物细胞表达黄素- 含有单加氧酶的体外实验将有商业应用 异种生物的代谢和毒性分析。这一新系统 补充了现有的含有细胞色素P450酶的系统。

Rabbit FM01 and FM02 and human FM03 cDNAs will be expressed in AHH-1TK+/- human lymphoblastoid cells, AS52 Chinese hamster cells and C3H 10T1/2 mouse cells. FMO expression will be monitored by regio- and stereoselective/enzyme assays and immunoblotting. The level and stability of FMO expression will be monitored in the cDNA expressing cell lines. The differences in xenobiotic metabolism by allelic variants of human FM03 will be examined. The FMO-expressing human lymphoblastoid cell lines will compliment an existing panel of cell lines expressing cytochrome P450 enzymes. AS52 cells will allow the facile analysis of mutational spectrum for promutagens activated by FMO. The C3H 10T1/2 cell lines will allow extension of in vitro toxicology studies to the malignant transformation endpoint. PROPOSED COMMERCIAL APPLICATION: Mammalian cells expressing flavin- containing monoxygenases will have commercial applications for the in vitro analysis of xenobiotic metabolism and toxicity. This new system complements an existing system containing cytochrome P450 enzymes.