DEVELOPMENT OF CELL LINES EXPRESSING OXYGEN GENERATORS

这项拟议的研究的目的是在体外培养哺乳动物细胞， 它们会产生过量的氧自由基，因此可以作为模型 氧自由基效应的研究系统及其防护方法 对抗氧自由基损伤。这些细胞系将由 构建含氧自由基编码基因的载体 产生酶，然后将载体导入人体 淋巴母细胞系AHH-1。这些DNA将在 可调节的促进剂，使氧自由基的产生水平可以 受到监管。然后，这些细胞系将被用来研究 氧自由基在各种细胞过程中的作用，包括DNA损伤和 确定可防止氧自由基损伤的试剂。

The object of the proposed research is to develop mammalian cells in vitro, which overproduce oxygen radicals and therefore can be used as model systems for studying oxygen radical effects and methods of protecting against oxygen radical damages. The cell lines will be developed by constructing vectors containing cDNAs which code for oxygen radical producing enzymes and then transfecting the vectors into the human lymphoblastoid cell line AHH-1. The cDNAs will be under the control of regulatable promoters so that the levels of oxygen radical production can be regulated. These cell lines will then be used to study the effects of oxygen radicals on various cellular processes, including DNA damage and to identify agents which protect against oxygen radical damage.