DEVELOPMENT OF CACO-2 CELLS EXPRESSING CYTOCHROME P450

表达细胞色素 P450 的 CACO-2 细胞的发育

• 批准号: 2023002
• 负责人: CHARLES L CRESPI
• 金额: $ 24.12万
• 依托单位: GENTEST CORPORATION
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-12-15 至 1999-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2023002
• 关键词: DEVELOPMENT; CACO; CELLS; EXPRESSING; CYTOCHROME

We propose to evaluate the feasibility of expressing human cytochrome P450 (CYP) cDNAs in Caco-2 human intestinal epithelial cells, a model system for the study of drug/xenobiotic uptake and transport. Expression studies will be conducted using CYP2A6 as a convenient and appropriate model enzyme and then extended to CYP3A4, the major cytochrome P450 in human intestinal epithelium. The suitability of transfected cells for studies of xenobiotic transport will also be evaluated. The overall goal is to develop Caco-2 cell derivatives which express the human cytochrome P450s found in the intestine in vivo in order to establish a better in vitro model system for drug transport / metabolism and for detecting the potential therapeutically relevant drug-drug or drug-nutrient interactions. PROPOSED COMMERCIAL APPLICATION: An improved in vitro model system to study drug metabolism and uptake. A useful system to help choose which drug candidates should be developed.

我们建议评估表达人细胞色素的可行性