ENHANCER/PROMOTER INTERACTIONS IN THE BITHORAX COMPLEX

拟议研究的目标是了解以下基本原则 特殊复杂基因座中增强子-启动子的相互作用 强调果蝇双胸复合体(BX-C)的ABD-B区。 Abd-B基因包含5个段旁特异的调节区，或 腹下(IAB)区域，跨越大约100 kb的DNA。 两类特殊的DNA元件可能调节增强子- 启动子相互作用和保持邻近顺式调控区域 分离；增强子DNA可能促进增强子-启动子相互作用 在很长的距离上。绝缘体DNA元件，前腹-7(FAB-7)， 其特征是介于IAB-6和IAB-7之间。《公约》的具体目标 建议的研究是通过以下方式确定Fab-7的反式作用因素 基因筛选，并识别和表征具有 促进长程增强子启动子的相互作用。

The goal of the proposed studies is to understand the basic principles of enhancer-promoter interactions in complex genetic loci with particular emphasis of the Abd-B region of the Drosophila bithorax complex (BX-C). The Abd-B gene contains 5 parasegmental-specific regulatory regions, or infra abdominal (iab) regions that span over approximately 100 kb of DNA. Two class of specialized DNA elements may might regulate enhancer- promoter interaction and to keep neighboring cis regulatory regions separates; augmenter DNA might facilitate enhancer-promoter interaction over long distances. An insulator DNA element, Front abdominal-7 (Fab-7), has been characterized between iab-6 and iab-7. The specific aims of the proposed study is to identify trans-acting factors for Fab-7 through genetic screens, and to identify and characterize augmenter elements that facilitate long range enhancer promoter interactions.