ENHANCER/PROMOTER INTERACTIONS IN THE BITHORAX COMPLEX

拟议研究的目标是了解 增强子-启动子相互作用在复杂的遗传基因座， 果蝇双胸复合体（BX-C）的Abd-B区域的强调。 Abd-B基因含有5个副节段特异性调控区，或 腹下（iab）区域，其跨越大约100 kb的DNA。 两类特殊的DNA元件可能调节增强子- 启动子相互作用和保持相邻的顺式调节区 增强子DNA可能促进增强子-启动子相互作用 在很长的距离。绝缘子DNA元件，前腹-7（Fab-7）， 在IAB-6和IAB-7之间表征。的具体目标 拟议的研究是通过以下途径鉴定Fab-7的反式作用因子： 基因筛选，并确定和表征增强因子元件， 促进长距离增强子-启动子相互作用。

The goal of the proposed studies is to understand the basic principles of enhancer-promoter interactions in complex genetic loci with particular emphasis of the Abd-B region of the Drosophila bithorax complex (BX-C). The Abd-B gene contains 5 parasegmental-specific regulatory regions, or infra abdominal (iab) regions that span over approximately 100 kb of DNA. Two class of specialized DNA elements may might regulate enhancer- promoter interaction and to keep neighboring cis regulatory regions separates; augmenter DNA might facilitate enhancer-promoter interaction over long distances. An insulator DNA element, Front abdominal-7 (Fab-7), has been characterized between iab-6 and iab-7. The specific aims of the proposed study is to identify trans-acting factors for Fab-7 through genetic screens, and to identify and characterize augmenter elements that facilitate long range enhancer promoter interactions.