HOMEODOMAIN PROTEIN CUT DURING OOGENESIS

同源域蛋白质在卵发生过程中被切割

• 批准号: 2634880
• 负责人: STEPHEN M JACKSON
• 金额: $ 3.09万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-16 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2634880
• 关键词: DNA binding protein; Drosophilidae; developmental genetics; gene expression; gene mutation; oogenesis; tissue mosaicism

The cut locus of Drosophila encodes a homeodomain protein (Cut) that controls cell fates in several embryonic and adult tissues. In this application, experiments are proposed that characterize the role of cut during oogenesis, a developmental system in which many changes in cellular physiology occur and is amenable to molecular and genetic manipulation. Preliminary mosaic analysis using the FLP/FRT system and analysis of hypomorphic combinations of cut alleles has shown that decreased cut expression results in altered organization of the germline-derived cells and eventual degradation of follicles. Conversely, ectopic Cut protein expression results in follicles that contain too many germline-derived cells, and later disrupts formation of the dorsal cuticle in progeny of these females. Thus, consistent with its biphasic expression pattern in the ovary, cut appears to a formation of follicles in early stages, and maternal Cut expression influences pattern formation during embryogenesis. Presumably, this is accomplished by influencing cell fate choices and/or by influencing patterns of gene expression. The role of cut in the formation of individual follicles will be determined by examining effects of altered Cut expression on specific follicle cell fates. interactions between cut and genes that pro similar oogenic disruptions when mutated will be examined. The maternal contribution of cut will be determined by examining the effects of altered maternal Cut expression on the expression of genes that are involved in dorsal-central axis formation. To address the molecular mechanisms responsible for these effects, differential display will be used to isolate gene products that are regulated by Cut during oogenesis. These studies will provide insights into the role of the cut locus in influencing gene expression patterns and cell fate choices.

果蝇的cut基因座编码同源结构域蛋白（Cut）， 在几种胚胎和成人组织中控制细胞命运。在这 应用中，实验提出，特点切的作用 在卵子发生过程中，一个发育系统，其中细胞的许多变化， 生理学发生，并服从于分子和遗传操纵。 使用FLP/FRT系统进行初步镶嵌分析， 切割等位基因的亚纯型组合已经表明， 表达导致生殖系来源的细胞的组织改变 最终导致卵泡退化相反，异位切割蛋白 表达导致卵泡中含有太多的种系来源的 细胞，后来破坏了背角质层的形成，在后代的 这些女性。因此，与其在大肠杆菌中的双相表达模式一致， 卵巢切割后在早期阶段似乎有卵泡形成，并且 母体切割表达影响胚胎发生过程中的图案形成。 据推测，这是通过影响细胞命运选择和/或 通过影响基因表达模式。cut的作用 单个卵泡的形成将通过检查 特定卵泡细胞命运上的改变的Cut表达。相互作用 cut和突变时产生类似卵子破坏的基因之间的关系 将被审查。母体对cut的贡献将由以下因素决定： 检查改变的母体Cut表达对表达的影响， 背中轴形成的基因。解决 负责这些影响的分子机制，差异 展示将用于分离受切割调控的基因产物 在卵子发生过程中。这些研究将提供深入了解的作用 切割位点影响基因表达模式和细胞命运选择。