ABSORPTION OF ELLAGITANNINS FROM DIETARY SOURCES

从膳食中吸收鞣花单宁

• 批准号: 2012139
• 负责人: DANIEL W NIXON
• 金额: $ 3.89万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-15 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2012139
• 关键词: cancer prevention; chemical structure function; chemoprevention; clinical research; dietary constituent; fruit; gastrointestinal nutrient absorption; high performance liquid chromatography; human subject; infrared spectrometry; metabolism; nuclear magnetic resonance spectroscopy; nutrition aspect of cancer; nutrition related tag; pharmacokinetics; serology /serodiagnosis; tannins; urinalysis

DESCRIPTION: The long term goal of this project is to determine if hydrolyzable ellagitannins present in dietary sources prevent cancer in humans. The more immediate purpose of this project is to assess the a b s o r ption and metabolism of hydrolyzable ellagitannins present in raspberries, strawberries and walnuts by the human digestive system. We believe that the hydrolyzable ellagitannins are absorbed into the human blood stream and metabolized after raspberries, strawberries and walnuts are consumed. We also believe that these ellagitannins and metabolites can be identified and quantified. The purpose for doing this is to identify biologically active ellagitannins and metabolites for further cell and animal chemoprevention studies. This study will also identify which dietary source delivers the highest concentration of biologically active ellagitannins to the blood stream, which will be useful for continuing human chemoprevention clinical trials. In order to identify and quantify the ellagitannins absorbed into the blood stream after raspberries, strawberries, and walnuts are consumed, thirty healthy volunteers will be broken into three equal groups and admitted to the MUSC General Clinical Research Center. After baseline urine and serum samples are obtained, the volunteers will be fed a known quantity of either raspberries, strawberries or walnuts under controlled conditions. Urine will be collected and a serial blood draw will be taken from each volunteer over the 24-hour study period. Known ellagitannins will then be identified and quantified in the blood and urine by HPLC. Unknown ellagitannins and ellagitannin metabolites will be isolated by HPLC and submitted for Mass Spectrum (FAB), NMR, UV and FR-IR analysis so that structural determinations can be made.

描述：该项目的长期目标是确定是否 饮食来源中存在的可水解伊拉吉尼蛋白可预防癌症 人类。 该项目的更直接目的是评估a b s o 覆盆子中存在的可水解伊拉吉丁蛋白的r pt和代谢， 人类消化系统的草莓和核桃。 我们相信 可水解的伊拉吉丁素被吸收到人类血流中， 消耗覆盆子，草莓和核桃后代谢。 我们 还认为可以识别这些Ellagitannins和代谢产物 量化。 这样做的目的是识别生物学活跃 Ellagitannins和代谢物，用于进一步的细胞和动物化学预防 研究。 这项研究还将确定哪种饮食来源可提供 最高浓度的生物活性Ellagitannin到血液 流，这对于继续人类化学预防临床非常有用 试验。 为了识别和量化吸收到 消耗覆盆子，草莓和核桃后的血液， 三十名健康的志愿者将分为三组， 进入MUSC一般临床研究中心。 基线尿液后 并获得血清样品，将为志愿者提供已知数量的志愿者 在受控条件下，在覆盆子，草莓或核桃中。 将收集尿液，并从每个 在24小时学习期间的志愿者。 然后，已知的Ellagitannins将是 通过HPLC在血液和尿液中识别并定量。 未知 HPLC和 提交质谱（FAB），NMR，UV和FR-IR分析，以便 可以做出结构确定。