ABSORPTION OF ELLAGITANNINS FROM DIETARY SOURCES

描述：该项目的长期目标是确定 膳食来源中存在的可水解鞣质可预防癌症 人类。这个项目更直接的目的是评估a b S o 覆盆子中可水解性鞣质的吸收和代谢， 草莓和核桃被人体消化系统吸收。我们相信， 可水解性单宁被人体血液吸收，并 在食用覆盆子、草莓和核桃后代谢。我们 还认为这些鞣质和代谢物可以被识别和 量化的。这样做的目的是为了确定生物活性 用于进一步细胞和动物化学预防的鞣质和代谢物 学习。这项研究还将确定哪种饮食来源可以提供 血液中生物活性最高浓度的海藻单宁 Stream，这将对继续人类的化学预防临床有用 审判。为了鉴定和定量被吸收的海拉格丹宁 食用覆盆子、草莓和核桃后的血液流动， 30名健康志愿者将被分成三组，每组 被MUSC综合临床研究中心录取。基线后尿量 并获取血清样本，志愿者将获得已知数量的食物 在控制条件下的覆盆子、草莓或核桃。 将收集尿液，并对每个人进行连续抽血 在24小时的学习期间做志愿者。已知的单宁将会是 用高效液相色谱法对血液和尿液中的成分进行鉴定和定量。未知 利用高效液相色谱分离海带单宁和海带单宁代谢产物 已提交进行质谱分析、核磁共振、UV和FR-IR分析，以便 可以做出结构性决定。

DESCRIPTION: The long term goal of this project is to determine if hydrolyzable ellagitannins present in dietary sources prevent cancer in humans. The more immediate purpose of this project is to assess the a b s o r ption and metabolism of hydrolyzable ellagitannins present in raspberries, strawberries and walnuts by the human digestive system. We believe that the hydrolyzable ellagitannins are absorbed into the human blood stream and metabolized after raspberries, strawberries and walnuts are consumed. We also believe that these ellagitannins and metabolites can be identified and quantified. The purpose for doing this is to identify biologically active ellagitannins and metabolites for further cell and animal chemoprevention studies. This study will also identify which dietary source delivers the highest concentration of biologically active ellagitannins to the blood stream, which will be useful for continuing human chemoprevention clinical trials. In order to identify and quantify the ellagitannins absorbed into the blood stream after raspberries, strawberries, and walnuts are consumed, thirty healthy volunteers will be broken into three equal groups and admitted to the MUSC General Clinical Research Center. After baseline urine and serum samples are obtained, the volunteers will be fed a known quantity of either raspberries, strawberries or walnuts under controlled conditions. Urine will be collected and a serial blood draw will be taken from each volunteer over the 24-hour study period. Known ellagitannins will then be identified and quantified in the blood and urine by HPLC. Unknown ellagitannins and ellagitannin metabolites will be isolated by HPLC and submitted for Mass Spectrum (FAB), NMR, UV and FR-IR analysis so that structural determinations can be made.