PATTERNS OF HIV-1 GENETIC VARIATION OVER TIME IN EIGHT PATIENTS
PATTERNS OF HIV-1 GENETIC VARIATION OVER TIME IN EIGHT PATIENTS
批准号:
2463687
负责人:
C A WINKLER
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
关键词:
HIV envelope protein HIV infections biochemical evolution clinical research complementary DNA genetic mapping genetic polymorphism helper T lymphocyte hemophilia As human immunodeficiency virus 1 human tissue immunogenetics leukocyte count nucleic acid sequence polymerase chain reaction virion virus RNA virus characteristic virus genetics
中文摘要
在纵向系统发育分析中检查了HIV-1变异
英文摘要
HIV-1 variation was examined in a longitudinal phylogenetic analysis
of the V3 region of the HIV env gene in 8 hemophiliac children and
adolescents. The patients were selected based on the rate of CD4 T-
cell decline during the first two years of enrollment in the Hemophilia
Growth and Development Study. The analysis differs from most previous
studies in that plasma HIV viral RNA and not proviral DNA was analyzed
for the extent and character of virion diversity during the latent
asymptomatic period through CD4 T-cell decline. Major preliminary
findings are: (1) Individuals with similar clinical courses have
distinctively different levels of viral diversity during the period of
CD4 T-cell decline, suggesting differential immune pressures driving
the rate of quasispecies evolution. (2) Two divergent populations of
HIV in a single patient, one with macrophage- and the other
T-cell-tropic genotypic features, co-existing over multiple timepoints
and with different rates of evolution were detected. (3) Positive
selection for amino acid substitution within the V3 region correlates
well with immune status. In three of the four patients, there was a
general trend towards positive selection for change when CD4 T-cell
counts were >200, but mutations tend to be silent when immune pressure
decreased as CD4 T-cell counts fell below 200. (4) Sequences from
multiple timepoints within a patient did not cluster in distinct
clades. Instead intra-timepoint sequences were found to overlap with
sequences from other timepoints. This suggests that early variants
persist over time and may represent escape mutants that evaded immune
surveillance.
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会议论文
SDF-1 3'UTR MUTATION DELAYS PROGRESSION TO AIDS
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批准号:6101054
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:C A WINKLER
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依托单位:
IDENTIFICATION OF GENETIC MARKERS FOR FOCAL SEGMENTAL GLOMERULOSCLEROSIS
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批准号:2463820
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:C A WINKLER
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依托单位:
EVOLUTION OF HIV 1 IN SIX HEMOPHILIAC CHILDREN
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批准号:6160967
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:C A WINKLER
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依托单位:
IDENTIFICATION OF GENETIC MARKERS FOR FOCAL SEGMENTAL GLOMERULOSCLEROSIS
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批准号:6101014
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:C A WINKLER
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依托单位:
SDF-1 3'UTR MUTATION DELAYS PROGRESSION TO AIDS
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批准号:6161154
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项目类别:
-
资助金额:$0.0万
-
财政年份:--
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负责人:C A WINKLER
-
依托单位:
IDENTIFICATION OF GENETIC MARKERS FOR FOCAL SEGMENTAL GLOMERULOSCLEROSIS
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批准号:6161114
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:C A WINKLER
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依托单位:
海外基金