EVOLUTION OF HIV 1 IN SIX HEMOPHILIAC CHILDREN

用纵向系统发育分析方法研究了HIV-1的进化 6例血友病患者HIV env基因V3区序列分析 儿童和青少年。参与者是根据 CD 4 T细胞下降的轨迹在以下的头两年 研究入组。主要的发现是， 临床病程具有明显不同的病毒多样性水平 在通过CD 4 T细胞下降的潜伏无症状期期间， 这表明不同的免疫压力推动了 准种进化两个不同的艾滋病毒种群在一个 患者，一个具有巨噬细胞，另一个具有嗜T细胞基因型 在多个时间点观察到这些特征， 不同层次的多样性。T-热带分支趋向于 增加碱性残基的积累，并可能具有更高的亲和力 CXCR 4受体。我们也证实了其他人的发现 有一个强烈的趋势，积极选择的变化时， CD 4 T细胞计数大于200。

The evolution of HIV-1 was examined by longitudinal phylogenetic analysis of the V3 region of the HIV env gene in six hemophiliac children and adolescents. The participants were selected based on the trajectory of CD4 T-cell decline during the first two years following study enrollment. Major findings were that individuals with similar clinical courses have distinctively different levels of viral diversity during the latent asymptomatic period through CD4 T-cell decline, suggesting differential immune pressures driving the rate of quasispecies evolution. Two divergent populations of HIV in a single patient, one with macrophage- and the other with T-cell-tropic genotypic features, were observed co-existing over multiple timepoints with different levels of diversity. The T-tropic clades trended towards an increasing accumulation of basic residues and may have a higher affinity for the CXCR4 receptor. We have also confirmed the findings of others that there is a strong trend towards positive selection for change when CD4 T-cell counts were greater than 200.