PRECLINICAL SAFETY AND IMMUNOGENICITY OF GROUP B MENINGOCOCCAL VACCINES

不同抗原诱导的恒河猴抗体结合特异性 B群脑膜炎奈瑟菌结合疫苗的研究 使用多种B组脑膜炎双球菌多糖(GBP)抗原 系统。通过酶联免疫吸附试验检测，抗体反应显著。 比含间隔基团的Gbps或N-丙酰化版本的Gbps更高 而不是与甲基化人血清白蛋白络合的天然GBPS (HSA)。针对血吸虫病毒的一组抗体的特异性 间隔区ADH通过它们与一种无关肺炎球菌的结合得到证实 9V多糖通过ADH与人血清白蛋白偶联。由日本血吸虫引起的抗体 GBP-OMV结合疫苗也显示与N-丙酰化GBP结合 与人血清白蛋白结合。作为上一次免疫的延伸 按照计划，用纯化的疫苗进一步免疫四组猴子 50周时仅Gbps。对纯Gbps没有助推器反应。 对血清进行杀菌抗体检测。使用人类 补充，疫苗不会引起杀菌抗体的增加 针对B组多糖决定簇。然而， 含有外膜囊泡的结合物，但不含未结合的 水泡控制疫苗诱导高水平杀菌抗体 针对内毒素决定因素。

The binding specificity of rhesus antibodies elicited by various conjugate vaccines of group B Neisseria meningitidis (GBNM) was studied using a variety of group B meningococcal polysaccharide (GBPS) antigen systems. The antibody response, as measured by ELISA, was significantly higher to the spacer-containing GBPS or N-propionylated version of GBPS than to the native GBPS complexed with methylated human serum albumin (HSA). The specificity of a population of antibodies directed to the spacer, ADH, was confirmed by their binding to an unrelated pneumococcal 9V polysaccharide conjugated to HSA through ADH. Antibodies elicited by GBPS-OMV conjugate vaccine also showed binding to N-propionylated GBPS conjugated to HSA. As an extension of the previous immunization schedule, four groups of monkeys were further immunized with the purified GBPS alone at week 50. There was no booster response to the pure GBPS. The sera were examined for bactericidal antibodies. Using human complement, the vaccines induced no increases in bactericidal antibody directed against group B polysaccharide determinants. However, the conjugates containing outer membrane vesicles, but not the unconjugated vesicle control vaccine induced high levels of bactericidal antibodies directed at lipopolysaccharide determinants.