TRICHOSPORON BIEGELII PS CROSS REACTS WITH CRYPTOCOCCAL CAPSULAR ANTIGEN

Trichosporon BIEGELII PS 与隐球菌荚膜抗原发生交叉反应

• 批准号: 3748115
• 负责人: S DEVI
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3748115
• 关键词: Cryptococcus neoformans; Fungi; antibody specificity; antifungal antibody; antiserum; carbohydrate structure; cell capsule; fungal antigens; genetic strain; immunoelectrophoresis; polysaccharides

Trichosporon beigelii is an opportunistic fungus which is being isolated in recent years from fatal septicemic infections in immunocompromised patients, particularly in those with cancer and granulocytopenia. Serodiagnosis of this condition is often made using a cryptococcal latex agglutination test, because of the cross-reaction between a cell wall polysaccharide antigen of T. beigelii and the capsular glucuronoxylomannan of Cryptococcus neoformans, another fatal opportunistic fungal pathogen. We isolated, purified and immunochemically characterized the cross-reactive T. beigelii polysaccharide. Its repeat unit consists of mannose, xylose, glucuronic acid and glucose; the polysaccharide is O-acetylated. We also perfirmed a comparative quantitation of this polysaccharide in clinical and non- clinical isolates of T. beigelii recovered from different parts of the U.S. using rocket immunoelectrophoresis. Both clinical and non-clinical strains produced the polysaccharide in vitro; significantly higher quantity of the polysaccharide was released by the deep isolates than by superficial isolates.C. neoformans is an invasive fungal pathogen and the capsular glucuronoxylomannan (GXM) of all serotypes of C. neorormans are O-acetylated, whereas that of less virulent cryptococci such as C. laurentii and C. albidus are non-O-acetylated. Using an immunodot technique and an O-acetyl-specific cryptococcal antiserum, we found a significantly higher percentage (89%) of deep isolates of T. beigelii produce O-acetylated polysaccharide, compared to only 17% of superficial isolates. Thus, the cross-reactive polysaccharide antigen of T. beigilii serves as a diagnostic marker and may play a role in pathogenesis.

Beigelii丝孢酵母是一种正在分离的机会致病真菌 近年来，在免疫功能低下的患者中， 患者，特别是癌症和粒细胞减少症患者。 这种情况的血清学诊断通常使用隐球菌乳胶 凝集试验，由于细胞壁之间的交叉反应， T.贝格利和囊膜 葡萄糖醛酸甘露聚糖的新型隐球菌，另一个致命的 机会致病真菌 我们分离，纯化， 免疫化学特征的交叉反应T。贝格利 多糖 其重复单元由甘露糖、木糖、葡萄糖醛酸组成 酸和葡萄糖;多糖是O-乙酰化的。 我们还完善了 在临床和非临床中对这种多糖进行比较定量， 临床分离的T.从不同的地方发现的beigelii 美国使用火箭免疫电泳。 临床和非临床 菌株在体外产生的多糖;显着高于 深层分离物的多糖释放量高于深层分离物 浅层分离物C.新生菌是一种侵袭性真菌病原体， 荚膜葡糖醛酸甘露聚糖（GXM）的所有血清型的C.内奥尔曼群岛 O-乙酰化，而毒性较小的隐球菌如C. laurentii和C. albidus是非-O-乙酰化的。 使用免疫点 技术和O-乙酰特异性隐球菌抗血清，我们发现了一个 深层分离株占总分离株的89%。贝格利 生产O-乙酰化多糖，相比之下，只有17%的表面 分离株 因此，T.贝吉利 作为诊断标志物，并可能在发病机制中发挥作用。