TRICHOSPORON BIEGELII PS CROSS REACTS WITH CRYPTOCOCCAL CAPSULAR ANTIGEN

Trichosporon BIEGELII PS 与隐球菌荚膜抗原发生交叉反应

• 批准号: 5200681
• 负责人: S DEVI
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5200681
• 关键词: Cryptococcus neoformans; Fungi; antifungal antibody; carbohydrate structure; cell capsule; cross immunity; epitope mapping; fungal antigens; immunoprecipitation; microorganism immunology; monoclonal antibody; polysaccharides; virulence

The cell wall polysaccharide of Trichosporon beigelii that cross-reacts with the capsular glucuronoxylomannan of Cryptococcus neoformans was further characterized immunologically. Previously, among other epitopes, we had established the presence of glucuronic acid and O-acetyl epitopes on the T. beigelii polysaccharide by NMR and chemical means. We have confirmed the identity of T. beigelii polysaccharide with the glucuronoxylomannan of C. neoformans with reference to these two important epitopes by using two monoclonal antibodies to the glucuronyl and O-acetyl epitopes of the cryptococcal glucuronoxylomannan (a gift from Dr. T. Kozel, University of Nevada, Las Vegas). Both the polysaccharides showed an identical pattern of precipitation with the two monoclonal antibodies. In C. neoformans, both glucuronyl and O-acetyl epitopes are closely associated with virulence, immunogenicity and biological activity. Our previous demonstration of a good correlation between the amount of polysaccharide present in deep-seated isolates and the degree of resistance to phagocytosis along with the current results suggest the possible role of T. beigelii polysaccharide in pathogenesis of septicemia and other deep-seated infections.

交叉反应的贝氏丝孢酵母细胞壁多糖 与新型隐球菌的荚膜葡萄糖醛酸甘露聚糖的结合， 免疫学上的特征。 以前，在其他表位中， 我们已经确定了葡萄糖醛酸和O-乙酰基表位的存在 在T。通过核磁共振和化学方法研究了葛根多糖。 我们有 确认了T.葛根多糖 葡糖醛酸甘露聚糖C.关于这两个新形式 使用两种针对葡萄糖醛酸基的单克隆抗体研究重要表位 和隐球菌葡糖醛酸甘露聚糖的O-乙酰基表位（一种礼物， T博士的Kozel，内华达州大学，拉斯维加斯）。 两者 多糖的沉淀模式与两者相同 克隆抗体 In C.新型葡萄糖醛酸和O-乙酰 表位与毒力、免疫原性和 生物活性 我们之前展示的良好相关性 存在于深层分离物中的多糖的量与 对吞噬作用的抵抗程度沿着当前结果 提示T.葛根多糖的致病作用 败血症和其他深层次感染。