TRICHOSPORON BIEGELII PS CROSS REACTS WITH CRYPTOCOCCAL CAPSULAR ANTIGEN

具有交叉反应能力的毕氏毛孢子菌细胞壁多糖 用新型隐球菌囊膜葡糖醛酸木甘露聚糖 进一步表现出免疫学特征。此前，在其他表位中， 我们已经确定了葡萄糖醛酸和O-乙酰表位的存在 用核磁共振和化学方法对甜瓜多糖进行了研究。我们有 证实了贝氏毛滴虫多糖的同源性 新生隐球菌葡糖醛酸木甘露聚糖与这两种物质的关系 利用两株抗葡萄糖醛酸基的单抗确定重要表位 隐球菌葡糖醛酸氧基甘露聚糖的O-乙酰基表位(礼物 来自拉斯维加斯内华达大学的T.Kozel博士)。这两个 多糖表现出与两者相同的沉淀模式 单克隆抗体。在新生葡萄球菌中，葡萄糖醛酸基和O-乙酰基都是 表位与毒力、免疫原性和 生物活性。我们之前展示的良好的相关性 在深层分离物中存在的多糖量和 对吞噬作用的抵抗程度以及目前的结果 提示贝氏毛滴虫多糖在发病机制中的可能作用 败血症和其他深层次感染。

The cell wall polysaccharide of Trichosporon beigelii that cross-reacts with the capsular glucuronoxylomannan of Cryptococcus neoformans was further characterized immunologically. Previously, among other epitopes, we had established the presence of glucuronic acid and O-acetyl epitopes on the T. beigelii polysaccharide by NMR and chemical means. We have confirmed the identity of T. beigelii polysaccharide with the glucuronoxylomannan of C. neoformans with reference to these two important epitopes by using two monoclonal antibodies to the glucuronyl and O-acetyl epitopes of the cryptococcal glucuronoxylomannan (a gift from Dr. T. Kozel, University of Nevada, Las Vegas). Both the polysaccharides showed an identical pattern of precipitation with the two monoclonal antibodies. In C. neoformans, both glucuronyl and O-acetyl epitopes are closely associated with virulence, immunogenicity and biological activity. Our previous demonstration of a good correlation between the amount of polysaccharide present in deep-seated isolates and the degree of resistance to phagocytosis along with the current results suggest the possible role of T. beigelii polysaccharide in pathogenesis of septicemia and other deep-seated infections.