IMMUNE RESPONSE TO GROUP B MENINGOCOCCAL POLYSACCHARIDE

对 B 组脑膜炎球菌多糖的免疫反应

• 批准号: 3792319
• 负责人: S DEVI
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3792319
• 关键词: N acetylneuraminate; Neisseria meningitidis; antibacterial antibody; antibody formation; bacterial antigens; bacterial capsules; bacterial polysaccharides; conformation; enzyme linked immunosorbent assay; epitope mapping; human subject; human tissue; immunoglobulin G; immunoglobulin M; serum

Group B meningococcal capsular polysaccharide (GBPS) consists of a linear polymer of alpha (2->8) linked N-acetyl neuraminic acid. Immune response to GBPS in humans has not been studied in detail. Most of the assays in use modify the structure of native antigen or its conformation to varying extent. We adopted an ELISA to measure antibodies to both GBPS and Escherichia coli K1, 0-acetylated polysaccharide in sera of healthy normals and group B meningococcal patients and carriers. In general, non-carriers showed higher total immunoglobulin levels to 0-acetylated K1 CPS than to GBPS, whereas patients and carriers had more antibodies to GBPS. Both IgG and IgM capsular antibodies were found. Attempts are under way to standardize ELISA to quantitate antibodies and express results in an acceptable unitage. Both the coating antigens are being used in different forms to understand finer epitope specificities. We intend to investigate possible association of various epitopes with different biologic properties. The kinetics of antibodies induced in laboratory animals by GBPS conjugates with and without adjuvants are being studied.

B群脑膜炎球菌荚膜多糖（GBPS）由以下组成： α（2->8）连接的N-乙酰神经氨酸的线性聚合物。免疫 对GBPS在人体中的反应尚未进行详细研究。 大部分 使用中的测定改变天然抗原的结构或其构象 在不同程度上。我们采用ELISA法检测两种GBPS的抗体 和大肠杆菌K1，0-乙酰化多糖 健康正常人和B群脑膜炎球菌患者和携带者。在 一般来说，非携带者的总免疫球蛋白水平较高， 0-乙酰化K1 CPS比GBPS，而患者和携带者 更多的GBPS抗体 IgG和IgM荚膜抗体均为阴性。 found.目前正在尝试将ELISA标准化， 抗体和表达结果在一个可接受的不确定性。两者 包被抗原以不同的形式使用，以了解更精细的 表位特异性。 我们打算调查 具有不同生物学特性的各种表位。的动力学 GBPS结合物在实验室动物中诱导的抗体， 不含佐剂的情况正在研究中。