ROLE OF MORPHOGENETIC PROTEINS IN EMBRYOGENESIS AND TISSUE REGENERATION

形态发生蛋白在胚胎发生和组织再生中的作用

• 批准号: 2568985
• 负责人: M MOOS
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2568985
• 关键词: adenylate cyclase; bone development; bone regeneration; cartilage development; developmental genetics; developmental neurobiology; embryogenesis; growth factor; nervous system regeneration; neurogenesis; osteogenesis; protein purification; tissue /cell culture; vertebrate embryology

During the past year, Dr. Moos has published a description of a novel growth factor, Anti-Dorsalizing Morphogenetic Protein (ADMP) that exerts profound control over the formation of the body axis in development in vertebrates. This protein may provide important regulatory control over nervous system development. The major claims contained in a patent application relating to three other genes (the Cartilage-Derived Morphogenetic Proteins) have been allowed, and seven major biotechnology firms have applied to license them. Two of these proteins are specific to articular cartilage, and during the past year have proven to exert chondrogenic and osteogenic activities. As such, they are leading candidates for clinical applications in bone and joint repair, and for manipulation of autologous cells.Initial studies describing the role of Osteogenic Protein-1 in early vertebrate development were completed and submitted for publication. The salient findings were that this growth factor is a powerful inducer of ventral derivatives; the most striking such effect is a dramatic induction of blood tissue. Another gene, Frzb, originally isolated from mammalian articular cartilage in collaboration with Frank Luyten of NIDR has proven to be a secreted growth factor. Recent experiments in the laboratory have demonstrated that the protein can induce formation of muscle and nerve tissue, and also participates in formation of the body axis in vertebrate organisms. Further, it appears to function as a soluble inhibitor to Wnt growth factors in functional assays. A patent filing on this factor is anticipated shortly.Finally, an improved method for purification of the signal transducing enzyme adenylyl cyclase was published.

在过去的一年里，穆斯博士发表了一部小说的描述 生长因子，抗背侧化形态发生蛋白（ADMP） 发育过程中对身体轴形成的深刻控制 脊椎动物。这种蛋白质可能提供重要的调节控制 神经系统发育。专利中包含的主要权利要求 与其他三个基因（软骨衍生基因）相关的应用 形态发生蛋白）已被允许，七大生物技术 公司已申请许可。其中两种蛋白质具有特异性 关节软骨，并且在过去的一年中已被证明可以发挥作用 软骨形成和成骨活性。因此，他们正在领先 骨和关节修复临床应用的候选人，以及 自体细胞的操作。描述作用的初步研究 早期脊椎动物发育中的成骨蛋白-1已完成并 提交出版。显着的发现是，这种增长 因子是腹侧衍生物的强大诱导剂；最引人注目的 这种效果是血液组织的显着诱导。 另一个基因，Frzb， 最初是从哺乳动物关节软骨中合作分离出来的 NIDR 的 Frank Luyten 已证明它是一种分泌性生长因子。 最近的实验室实验表明，该蛋白质 可以诱导肌肉和神经组织的形成，并参与 脊椎动物身体轴的形成。此外，它 似乎作为 Wnt 生长因子的可溶性抑制剂发挥作用 功能测定。预计将就此因素申请专利 最后，一种改进的信号纯化方法 转导酶腺苷酸环化酶已发表。