ROLE OF MORPHOGENETIC PROTEINS IN EMBRYOGENESIS AND TISSUE REGENERATION
ROLE OF MORPHOGENETIC PROTEINS IN EMBRYOGENESIS AND TISSUE REGENERATION
批准号:
3770374
负责人:
M MOOS
金额:
$0.0万
依托单位:
--
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
关键词:
中文摘要
在过去的一年里,我们的工作重点是发现增长
英文摘要
During the past year, our efforts have focused on discovery of growth
factors related to the bone morphogenetic proteins using degenerate PCR.
These proteins are emerging as key determinants in the process of
specification of positional information during embryogenesis and have
assumed importance to the biotechnology community owing to their almost
certain therapeutic utility in a variety of clinical contexts, including
nonunion fractures and other orthopedic and reconstructive indications and
alveolar ridge augmentation. We have devoted the majority of our
attention to genes expressed during gastrulation and neurulation in
Xenopus and Brachydanio rerio, though we have also examined genomic DNA
from these species. We have found six gene fragments which probably
encode heretofore unknown proteins. We have decided to focus on two of
these which are most clearly distinct from known proteins. One of these
was found in human and bovine cDNA in addition to Xenopus and Brachydanio;
this gene demonstrates 95% amino acid identity and 80% nucleotide identity
between all four species in the mature expressed protein product. The
other shows a similar degree of conservation between Xenopus and
Brachydanio; mammalian genes have not yet been identified. We have
isolated lambdal gt11 cDNA clones by hybridization to the PCR
amplification products and anticipate sequencing them during the next few
weeks. Next, we will determine the spatial and temporal expression
patterns of the genes by hybridization in situ using whole mounts of
Xenopus embryos, and investigate their functions by injecting mRNAs
transcribed in vitro to induced their over-expression. We will also
attempt expression of the proteins in order to explore their effects on
differentiation and the expression of trans acting factors (such as the
homeobox gene forkhead) in isolated animal caps and to allow
identification of specific receptors. Ultimately, we hope this strategy
will allow construction of dominant negative genes that can be used to
determine the effects of loss of function.
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会议论文
PURIFICATION OF ADENYLYL CYCLASE
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批准号:3804709
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M MOOS
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依托单位:--
PRIMARY STRUCTURE ANALYSIS OF REGULATORY PROTEINS
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批准号:3792436
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M MOOS
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依托单位:--
PURIFICATION AND CHARACTERIZATION OF ADENYLYL CYCLASE
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批准号:3792430
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M MOOS
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依托单位:--
ROLE OF MORPHOGENETIC PROTEINS IN EMBRYOGENESIS AND TISSUE REGENERATION
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批准号:6101247
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M MOOS
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依托单位:--
PRIMARY STRUCTURE DETERMINATION OF SIGNAL TRANSDUCTION PROTEINS
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批准号:3804716
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M MOOS
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依托单位:--
ROLE OF MORPHOGENETIC PROTEINS IN EMBRYOGENESIS AND TISSUE REGENERATION
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批准号:3748214
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M MOOS
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依托单位:--
ROLE OF MORPHOGENETIC PROTEINS IN EMBRYOGENESIS AND TISSUE REGENERATION
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批准号:2568985
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M MOOS
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依托单位:--
PURIFICATION AND CHARACTERIZATION OF ADENYLYL CYCLASE
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批准号:3811071
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M MOOS
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依托单位:--
ROLE OF MORPHOGENETIC PROTEINS IN EMBRYOGENESIS AND TISSUE REGENERATION
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批准号:6161307
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M MOOS
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依托单位:--
ROLE OF MORPHOGENETIC PROTEINS IN EMBRYOGENESIS AND TISSUE REGENERATION
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批准号:5200772
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M MOOS
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依托单位:--
海外基金