PRIMARY STRUCTURE DETERMINATION OF SIGNAL TRANSDUCTION PROTEINS

信号转导蛋白的初级结构测定

• 批准号: 3804716
• 负责人: M MOOS
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3804716
• 关键词: 2'3' cyclic nucleotide phosphodiesterase; 3'5' cyclic nucleotide phosphodiesterase; DNA binding protein; Dictyostelium; biological signal transduction; calmodulin; cartilage; cell adhesion molecules; chemical binding; electrophoresis; glycoproteins; phosphoproteins; protein sequence; protein structure

Several improvements in sequencer chemistry and supports have facilitated primary structure determination of two cyclic nucleotide phosphodiesterase, including identification of the cGMP binding site of one of the enzymes, a hormonally-regulated phosphoprotein associated with the adipocyte fat droplet, a protein specifically expressed in regenerating optic nerve, a novel bone morphogenetic protein isolated from mammalian cartilage, adhesion molecules from two oral pathogens, the nucleotide binding site of human platelet glycoprotein IIb, the Dictyostelium homolog of the GroEL protein, and a heretofore undescribed calmodulin binding protein from mammalian brain. Subpicomol determinations are now routine; we have recently obtained the majority of a protein's primary structure from a few microbrams of electrophoretically-separated material in one of the cases mentioned above. These procedures open the door to analysis of low- abundance polypeptides; further work will be directed toward identification and structure determination of additional proteins important in biological signal transduction.

测序仪化学和支持物的几项改进促进了 二环核苷酸磷酸二酯酶的一级结构测定， 包括鉴定一种酶的cGMP结合位点， 与脂肪细胞脂肪相关的经尿道调节的磷蛋白 液滴，一种在再生视神经中特异性表达的蛋白质， 从哺乳动物软骨分离的新的骨形态发生蛋白， 两种口腔病原体的粘附分子， 人血小板糖蛋白IIb，GroEL的网骨藻同系物 蛋白质，和迄今未描述的钙调蛋白结合蛋白， 哺乳动物的大脑亚皮摩尔测定现在是常规的;我们有 最近从一些蛋白质中获得了蛋白质一级结构的大部分 在其中一个案例中， 上面提到的。 这些程序打开了分析低- 丰富的多肽;进一步的工作将针对鉴定 生物学中重要的其他蛋白质的结构测定 信号转导