PURIFICATION AND CHARACTERIZATION OF ADENYLYL CYCLASE

腺苷酸环化酶的纯化和表征

• 批准号: 3792430
• 负责人: M MOOS
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3792430
• 关键词: adenylate cyclase; animal tissue; brain; guanine nucleotide binding protein; membrane; protein purification; protein sequence; protein structure function; western blottings

During the past year, the procedures developed previously were used to obtain adenylylcyclase preparations of unprecedented specific activity. Immunoblot analysis of this material using antibodies directed at defined sequences of two different cloned forms of the enzyme indicated that a majority of the enzymatic activity corresponded to a heretofore undescribed isoform. During the course of these studies, it was discovered that preactivation of the bovine brain membranes used as source material for the enzyme with a non-hydrolyzable GTP analog resulted in enhanced physical association between adenylylcyclase and the beta subunit of the stimulatory GTP binding protein, Gs. Manuscripts describing these findings have been completed and will be submitted shortly, and the project has been terminated.

在过去的一年里，以前开发的程序被用来 获得前所未有的比活性的腺苷酸环化酶制剂。 使用针对以下的抗体对该材料进行免疫印迹分析： 所示酶的两种不同克隆形式的确定序列 大多数酶活性对应于迄今为止的 未描述的同种型。 在这些研究的过程中，人们发现， 牛脑细胞膜作为酶的原料 与不可水解的GTP类似物，导致增强的物理 腺苷酸环化酶和β亚基之间的关联 刺激性GTP结合蛋白，Gs. 描述这些的手稿 调查结果已经完成，不久将提交， 项目已经终止。