PURIFICATION AND CHARACTERIZATION OF ADENYLYL CYCLASE

在过去的一年里，以前开发的程序被用来 获得比活性前所未有的腺苷环化酶制剂。 使用针对该材料的抗体进行免疫印迹分析 两种不同克隆形式的酶的定义序列表明 到目前为止，大多数酶的活性都与 未描述的异构体。 在这些研究过程中，人们发现预激活 作为酶的来源材料的牛脑膜 使用非水解性GTP类似物可增强物理性能 腺苷环化酶与血管紧张素转换酶β亚基的关系 刺激性GTP结合蛋白Gs。描述这些的手稿 调查结果已完成，并将于短期内提交， 项目已终止。

During the past year, the procedures developed previously were used to obtain adenylylcyclase preparations of unprecedented specific activity. Immunoblot analysis of this material using antibodies directed at defined sequences of two different cloned forms of the enzyme indicated that a majority of the enzymatic activity corresponded to a heretofore undescribed isoform. During the course of these studies, it was discovered that preactivation of the bovine brain membranes used as source material for the enzyme with a non-hydrolyzable GTP analog resulted in enhanced physical association between adenylylcyclase and the beta subunit of the stimulatory GTP binding protein, Gs. Manuscripts describing these findings have been completed and will be submitted shortly, and the project has been terminated.