EXPERIMENTS WITH A HIGH RESOLUTION FIELD EMISSION STEM

扫描透射电子显微镜(STEM)已与 平行电子能量损失谱(EELS)发展新的 补充传统电子显微镜技术的技术 结构生物学。评估价鳗鱼用于成像的潜力 生物化合物在细胞内的分布，高分辨率 从蛋白质、碳水化合物、 核酸和脂质，以及来自玻璃化的水。了解以下内容 这些价谱可以用来绘制细胞内水的分布。 冷冻的水合冰冻切片。改进的方法也被开发出来。 用于通过对通道间的校正来量化核心-边缘光谱 并通过减去主要的逆功率背景 核心边缘下的组件。光谱是从 具有良好特性的化合物，如半胱氨酸和三磷酸腺苷，从而允许 硫磺的相对散射截面有待测定， 磷和氮。

Scanning transmission electron microscopy (STEM) has been combined with parallel electron energy-loss spectroscopy (EELS) to develop new techniques that complement conventional electron microscopy-based structural biology. To assess the potential of valence EELS for imaging the distributions of biological compounds in cells, high-resolution spectra have been obtained from specimens of protein, carbohydrate, nucleic acid, and lipids, as well as from vitrified water. Knowledge of these valence spectra can be used to map cellular water distributions in frozen hydrated cryosections. Improved methods have also been developed for quantifying core-edge spectra by correcting for channel-to-channel gain variations and by subtracting the dominant inverse-power background component underlying the core-edges. Spectra have been recorded from well-characterized compounds such as cysteine and ATP, thus allowing relative scattering cross sections to be determined for sulfur, phosphorus, and nitrogen.