ANALYTICAL ELECTRON MICROSCOPY OF ADRENAL CHROMAFFIN CELLS

培养牛肾上腺的元素含量和水分含量 对嗜铬细胞及其分泌颗粒进行了测量 并与RAPID法分离的嗜铬颗粒进行比较 冷冻、超显微冷冻、X射线显微分析和电子技术 能量损失光谱学。一价离子的变化 分离的浓度被发现很大，而 钙和磷的浓度保持在大约 常量。利用测得的不同土壤水分含量 隔室，有可能估计一个阳性颗粒 膜电位(从内到外)约为10至16 MV.进行了额外的实验来测量变化 在培养基中加入氨的颗粒成分中 以引起体内PH值的变化。 用非弹性电子测定细胞的水分含量 用鳗鱼测量的散射量。该方法首先在 标准的水合冷冻切片，然后应用于 嗜铬细胞的不同隔室。

Elemental and water content of cultured bovine adrenal chromaffin cells and their secretory granules have been measured and compared with isolated chromaffin granules using rapid freezing, ultracryomicrotomy, x-ray microanalysis and electron energy loss spectroscopy. Changes in monovalent ion concentrations on isolation were found to be large whereas calcium and phosphorus concentrations remained approximately constant. Using measured water contents of different compartments it was possible to estimate a positive granule membrane potential (inside to outside) of approximately 10 to 16 mV. Additional experiments were performed to measure changes in granule composition by adding ammonia to the culture medium to induce changes in internal pH. Cellular water content was determined using inelastic electron scattering as measured by EELS. The method was first tested on hydrated cryosections of standards and was then applied to different compartments of the chromaffin cell.