ELEMENTAL MAPPING OF TRYPANOSOMA CRUZI BY ANALYTICAL ELECTRON MICROSCOPY

元素分析是从两个不同的克隆， 克氏锥虫（一种引起恰加斯病的致病原生动物） 疾病），以前已被证明有差异， 它们的毒性和光学性质。 这些分析是 通过BEIB中的元素映射完成 计算机化分析电子显微镜 样品 通过快速冷冻置于铵中的全细胞制备 硝酸盐缓冲液并负载在福尔马林/碳EM网格上; 样品在分析前是冷冻替代品。 X射线图 铁，锌，镁，钾，钙，磷，硫记录从一些 每个克隆的外鞭毛体。 不同的铁含量， 为两种细胞类型建立，一个克隆具有 大约是另一种铁含量的两倍， 似乎在线粒体区域， 归因于细胞色素。 其他差异也见于 二价阳离子（Mg、Ca和Zn）的量，其主要是 与磷酸盐结合，形成致密体， 细胞

Elemental analyses were obtained from two different clones of Trypanosoma Cruzi (a pathogenic protozoan causing Chagas' disease) that had previously been shown to have differences in their virulence and optical properties. The analyses was accomplished by means of elemental mapping in BEIB's computerized analytical electron microscope. Samples were prepared by rapidly freezing whole cells placed in an ammonium nitrate buffer and supported on formvar/carbon EM grids; samples were freeze-substitutes prior to analysis. X-ray maps of Fe, Zn, Mg, K, Ca, P, and S were recorded from a number of epimastigotes from each clone. Different iron contents were established for the two cell types, one clone having approximately twice the Fe content of the other, with most appearing to be in the mitochondrial region and possibly attributable to cytochromes. Other differences were seen in the amounts of divalent cations (Mg, Ca and Zn), which were mainly bound with phosphate in dense bodies scattered throughout the cells.