课题基金 / 基金详情

HEPATOCYTE MITOCHONDRIAL FUNCTION BY ANALYTICAL ELECTRON MICROSCOPY

HEPATOCYTE MITOCHONDRIAL FUNCTION BY ANALYTICAL ELECTRON MICROSCOPY
通过分析电子显微镜观察肝细胞线粒体功能
批准号：
3937355
负责人：
R D LEAPMAN
金额：
$0.0万
依托单位：
DIVISION OF RESEARCH SERVICES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3937355
关键词：
calcium metabolism cryoscience electron microscopy fatty acid metabolism laboratory rat liver cells liver metabolism membrane transport proteins mitochondria online computer tissue /cell preparation

项目摘要

项目成果

R D LEAPMAN的其他基金

相关文献

中文摘要
翻译
点击翻译按钮获取中文摘要
英文摘要
Mitochondrial free calcium in hepatocytes was estimated by treating rats with sodium butyrate. Metabolism of such short-chain fatty acids in mitochondria produces pyrophosphate and precipitates free calcium. Thin (80nm) cryosections of liver were sectioned using a cryoultramicrotome and transferred to an AEM for microanalysis. Dry wt. concentrations of calcium as well as diffusible ions (Na+, Mg++, K+, Cl-) were determined. Data were quantitated with software running on a Tractor Northern Analysis system and results were analyzed statistically on a Macintosh computer. Results support the view that there is high (approximately lmM) free calcium in the mitochondria.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
EXPERIMENTS WITH A HIGH RESOLUTION FIELD EMISSION STEM
MICROANALYSIS OF RAPIDLY-FROZEN TISSUE IN THE FIELD EMISSION STEM
BIOLOGICAL APPLICATIONS OF A COMPUTER CONTROLLED ANALYTICAL ELECTRON MICROSCOPE
  • 批准号：
    3959940
  • 项目类别：
  • 资助金额：
    $0.0万
  • 财政年份：
    --
  • 负责人：
    R D LEAPMAN
  • 依托单位：
    DIVISION OF RESEARCH SERVICES
ANALYTICAL ELECTRON MICROSCOPY OF ADRENAL CHROMAFFIN CELLS
  • 批准号：
    3959955
  • 项目类别：
  • 资助金额：
    $0.0万
  • 财政年份：
    --
  • 负责人：
    R D LEAPMAN
  • 依托单位：
    DIVISION OF RESEARCH SERVICES