DEVELOPMENT OF EMBRYO CRYOPRESERVATION PROCEDURES FOR LABORATORY RATS

实验大鼠胚胎冷冻保存程序的开发

• 批准号: 2590344
• 负责人: WILLIAM F. RALL
• 金额: --
• 依托单位: NATIONAL CENTER FOR RESEARCH RESOURCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2590344
• 关键词: animal breeding; animal population genetics; cryopreservation; embryo /fetus preservation; embryo /fetus transplantation; endocrine pharmacology; estrus; genetic registry /resource /referral center; gonadotropin releasing factor; laboratory rat; method development; tissue resource /registry; veterinary medicine

The NIH Animal Genetic Resource (NIHAGR) maintains many unique rat genotypes used as models of normal and disease processes in man. Effective management of this resource would be facilitated by the development of embryo cryopreservation procedures for long-term banking. In order to develop banks of cryopreserved germ plasm from laboratory rats, protocols for each step of the embryo banking and rederivation process must be established. These would include the preparation and mating of embryo donor females, embryo collection, cryopreservation, thawing, and embryo transfer. Previous studies suggest that superovulation procedures developed for laboratory mice are not as effective when applied to laboratory rats. The principal objective of this project is to evaluate treatment with an analog of gonadotropin releasing hormone (GnRH-a) as an alternative approach to synchronize estrus cycles for embryo collection and cryopreservation.

美国国立卫生研究院动物遗传资源（NIHAGR）保存了许多独特的大鼠 基因型被用作人类正常和疾病过程的模型。 有效管理这一资源将得到以下方面的促进： 制定长期储存胚胎的冷冻保存程序。 为了建立实验室超低温保存种质库， 大鼠，胚胎库和再衍生的每一步的方案 必须建立流程。 其中包括编制和 胚胎供体雌性交配，胚胎收集，冷冻保存， 解冻和胚胎移植。 先前的研究表明， 为实验室小鼠开发的超排卵程序并不像 对实验室老鼠有效。 的主要目标 这个项目是评估促性腺激素类似物的治疗效果 释放激素（GnRH-a）作为同步的替代方法 用于胚胎收集和冷冻保存的发情周期。