Development of Embryo Cryopreservation Procedures for Laboratory Rats

NIH动物遗传资源（NIHAGR） 保持了许多独特的大鼠基因型，用作正常和 人类疾病过程。有效管理这一资源 胚胎的发育 长期储存的冷冻保存程序。为了 从实验室大鼠中建立冷冻保存的种质库， 胚胎库和再衍生的每个步骤的方案 必须建立流程。其中包括准备 以及胚胎供体雌性的交配，胚胎收集， 冷冻保存、解冻和胚胎移植。以前的研究 表明为实验室开发超数排卵程序 当应用于实验室大鼠时，小鼠不那么有效。的 该项目的主要目标是评估治疗与 促性腺激素释放激素（GnRH-a）类似物作为 同步胚胎发情周期的替代方法 收集和冷冻保存。胚胎采集的效率 不同的模式之间的方式类似的育种 模型的性能。然而，在以下方面取得了进展： 来自所有大鼠模型的冷冻保存胚胎的开发库 检查到目前为止。

The NIH Animal Genetic Resource (NIHAGR) maintains many unique rat genotypes used as models of normal and disease processes in man. Effective management of this resource would be facilitated by the development of embryo cryopreservation procedures for long-term banking. In order to develop banks of cryopreserved germ plasm from laboratory rats, protocols for each step of the embryo banking and rederivation process must be established. These would include the preparation and mating of embryo donor females, embryo collection, cryopreservation, thawing, and embryo transfer. Previous studies suggest that superovulation procedures developed for laboratory mice are not as effective when applied to laboratory rats. The principal objective of this project is to evaluate treatment with an analog of gonadotropin releasing hormone (GnRH-a) as an alternative approach to synchronize estrus cycles for embryo collection and cryopreservation. The efficiency of embryo collection varies between models in a manner similar to that of the breeding performance of the model. However, progress has been made in developing banks of cryopreserved embryos from all rat models examined to date.