Development of Embryo Cryopreservation Procedures for Laboratory Rats

实验室大鼠胚胎冷冻保存程序的开发

• 批准号: 6112704
• 负责人: WILLIAM F. RALL
• 金额: --
• 依托单位: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6112704
• 关键词: cryopreservation; disease /disorder model; embryo /fetus cell /tissue; genetic registry /resource /referral center; genetic strain; genotype; gonadotropin releasing factor; laboratory rat; method development; technology /technique development; tissue /cell preparation; tissue resource /registry

The NIH Animal Genetic Resource (NIHAGR) maintains many unique rat genotypes used as models of normal and disease processes in man. Effective management of this resource would be facilitated by the development of embryo cryopreservation procedures for long-term banking. In order to develop banks of cryopreserved germ plasm from laboratory rats, protocols for each step of the embryo banking and rederivation process must be established. These would include the preparation and mating of embryo donor females, embryo collection, cryopreservation, thawing, and embryo transfer. Previous studies suggest that superovulation procedures developed for laboratory mice are not as effective when applied to laboratory rats. The principal objective of this project is to evaluate treatment with an analog of gonadotropin releasing hormone (GnRH-a) as an alternative approach to synchronize estrus cycles for embryo collection and cryopreservation. The efficiency of embryo collection varies between models in a manner similar to that of the breeding performance of the model. However, progress has been made in developing banks of cryopreserved embryos from all rat models examined to date.

美国国立卫生研究院动物遗传资源 (NIHAGR) 保留了许多独特的大鼠基因型，用作正常和 人类的疾病过程。有效管理该资源 将有利于胚胎的发育 长期储存的低温保存程序。为了 开发实验室大鼠冷冻种质库， 胚胎库和再衍生每个步骤的方案 必须建立流程。这些将包括准备工作 以及胚胎供体雌性的交配、胚胎收集、 冷冻保存、解冻和胚胎移植。之前的研究 建议为实验室开发超排卵程序 当应用于实验室大鼠时，小鼠的效果并不那么有效。这 该项目的主要目标是评估治疗方法 促性腺激素释放激素 (GnRH-a) 类似物 同步胚胎发情周期的替代方法 收集和冷冻保存。胚胎采集效率 模型之间的差异与育种类似 模型的性能。然而，已经取得了进展 开发来自所有大鼠模型的冷冻胚胎库 迄今为止已检查过。