DEVELOPMENT OF EMBRYO CRYOPRESERVATION PROCEDURES FOR LABORATORY RATS

实验大鼠胚胎冷冻保存程序的开发

• 批准号: 6290689
• 负责人: WILLIAM F. RALL
• 金额: --
• 依托单位: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6290689
• 关键词: cryopreservation; disease /disorder model; embryo /fetus cell /tissue; genetic registry /resource /referral center; genetic strain; genotype; gonadotropin releasing factor; laboratory rat; method development; technology /technique development; tissue /cell preparation; tissue resource /registry

The NIH Animal Genetic Resource (NIHAGR) maintains many unique rat genotypes used as models of normal and disease processes in man. Effective management of this resource would be facilitated by the development of embryo cryopreservation procedures for long-term banking. In order to develop banks of cryopreserved germ plasm from laboratory rats, protocols for each step of the embryo banking and rederivation process must be established. These would include the preparation and mating of embryo donor females, embryo collection, cryopreservation, thawing, and embryo transfer. Previous studies suggest that superovulation procedures developed for laboratory mice are not as effective when applied to laboratory rats. The principal objective of this project is to evaluate treatment with an analog of gonadotropin releasing hormone (GnRH-a) as an alternative approach to synchronize estrus cycles for embryo collection and cryopreservation. The efficiency of embryo collection varies between models in a manner similar to that of the breeding performance of the model. However, progress has been made in developing banks of cryopreserved embryos from all rat models examined to date. - rat embryo, cryopreservation, embryo bank, animal models

美国国立卫生研究院动物遗传资源(NIHAGR)保存了许多独特的大鼠基因类型，用于模拟人类的正常和疾病过程。为长期保存胚胎制定冷冻程序将有助于对这一资源的有效管理。为了开发实验室大鼠的超低温保存种质库，必须为胚胎库和再衍生过程的每一步建立方案。这些将包括胚胎捐赠者雌性的准备和交配、胚胎采集、冷冻保存、解冻和胚胎移植。以前的研究表明，为实验室小鼠开发的超排程序在应用于实验室大鼠时并不有效。本项目的主要目的是评价促性腺激素释放激素(GnRH-a)类似物作为胚胎采集和冷冻同步发情周期的替代方法的治疗效果。不同模型的胚胎收集效率不同，其方式类似于模型的繁殖性能。然而，到目前为止，在开发所有被检查的大鼠模型的冷冻保存胚胎库方面已经取得了进展。-大鼠胚胎、冷冻保存、胚胎库、动物模型