DEVELOPMENT & MAINTENANCE OF A CRYOPRESERVED EMBRYO BANK

发展

• 批准号: 2294171
• 负责人: WILLIAM F. RALL
• 金额: $ 13.01万
• 依托单位: SMITHSONIAN INSTITUTION
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-01 至 1998-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2294171
• 关键词: DEVELOPMENT; MAINTENANCE; CRYOPRESERVED; EMBRYO; BANK

The purpose of this contract is to establish and maintain a cryopreserved embryo bank of genetically defined laboratory rats and mice derived from the NIA colonies of aged rats and mice, which can be used to re-establish any particular strain should a genetic contamination or deviation be detected after rederivation of the NIA colonies, or a disease or environmental disaster occur. Similarly, should an investigator's results start deviating from their past norm after one or more rederivations have occurred, embryos from this stock would be used to try and determine if the evolution of effects seen was of animal or investigator origin. Prior to the cessation of entry of animals into each of the three currently established NIA aging rodent colonies, breeder males and females or pregnant female foundation stock will be sent to the Contractor for expansion to yield sufficient number of potential embryo donors.

本合同的目的是建立和维持一个 遗传学定义的实验室大鼠和小鼠的胚胎库， 老年大鼠和小鼠的NIA集落，可用于重建 任何特定的菌株，如果遗传污染或变异， 在NIA菌落重新衍生后检测到，或疾病或 环境灾难发生。 同样，如果调查人员的结果 在一个或多个重派生之后， 发生，胚胎从这个股票将被用来尝试和确定， 观察到的效应演变是动物或研究者的结果。 之前 停止动物进入目前三个 已建立的NIA老化啮齿动物群落，繁殖雄性和雌性，或 怀孕的女性基础股将被送往承包商， 扩增以产生足够数量的潜在胚胎供体。