TECHNOLOGY FOR CLINICAL MANAGEMENT OF OXIDATIVE STRESS

拟议的工作涉及三项相互关联的任务： 水杨酸盐激发试验对游离唾液酸标志物的水平和影响 在大约500人的群体中发生了激进的过程， 神经退行性疾病和对照。 鲁棒的发展 这些化合物用于研究和临床市场的方法， 色谱分离和电化学检测技术。 开发需求点技术， （谷胱甘肽）和氧化保护的一般指标。 新方法学发展的主要努力集中在硝化 氨基酸、DNA加合物和苯甲酸盐。 约60个其他指标 代谢状态（嘌呤、酪氨酸、色氨酸代谢物和 抗氧化剂维生素）将作为测试协变量进行监测。 目的是建立自由基激发试验的实用性， 临床评估中的标志物，并提供实验室和点- 需要检测技术才能有效利用。 拟议的商业应用：这项工作预计将产生新的 用于临床实验室氧化监测的检测和LC硬件 应激标记物和用于谷胱甘肽的即时一次性监测器 和氧化应激。

The proposed work involved three interrelated tasks: The study of the levels and effects of salicylate challenge tests on markers of free radical processes in a population of about 500 individuals with neurodegenerative disorders and controls. The development of robust methods for these compounds for the research and clinical market using new technologies for chromatographic separation and electrochemical detection. The development of point-of-need technologies for assessing specific (glutathione) and general indicators of oxidative protection. The principle new methodological developmental efforts focus on nitrated amino acids, DNA adducts, and benzoates. About 60 other indicators of metabolic status (purines, tyrosine, tryptophan metabolites, and antioxidant vitamins) will be monitored as test co-variables. The goals are to establish the utility of free radical challenge test and markers in clinical assessment, and to provide laboratory and point-of- need assay technology to utilize then effectively. PROPOSED COMMERCIAL APPLICATION: The work is expected to result in novel tests and LC hardware for clinical laboratory monitoring of oxidative stress markers and in point-of-need disposable monitors for glutathione and oxidative stress.