INTEGRATED COLUMN/MULTISENSOR MICROBORE SYSTEM

工作的长期目标是：1）开发一种低成本，高 灵敏度、高速模块化集成柱/多电化学 该系统适用于大多数现有的液相色谱泵， 电化学检测器;以及2）利用电化学检测器的速度和灵敏度， 系统开发更详细的了解内源性生化 过程和药理学机制。 II期研究将 针对：1）提高10 FG的灵敏度和分辨率， 第一阶段原型系统的30秒-3分钟间隔内的化合物; 2） 开发制造设计和协议以实现低成本 生产系统具有同等或更好的性能; 3）扩展 I期生物微透析工作证明实用性更好 神经元兴奋毒性模型基础研究中的时间分辨率 以及多巴胺能和多巴胺能系统的药理学; 4） 将应用领域扩展到不同的化合物组（氨基酸 和碳水化合物）和不同的市场（日本临床市场， 新生儿神经母细胞瘤中高香草酸和香草基扁桃酸含量测定 筛选）;以及5）启动理论和应用出版序列 为第三阶段的市场渗透奠定基础。

The long term objectives of the work are to: 1) develop a low cost, high sensitivity, high speed modular integrated column/multi-electrochemical system that is adaptable to most existing liquid chromatography pumps and electrochemical detectors; and 2) utilize the speed and sensitivity of the system to develop more detailed understanding of endogenous biochemical processes and pharmacological mechanisms. Phase II studies will be directed at: 1) improving the 10 fg sensitivities and resolution of compounds in 30 sec - 3 min intervals of the Phase I prototype systems; 2) developing the manufacturing designs and protocols to implement a low cost production system with equivalent or better performance; 3) extending the Phase I biological microdialysis work to demonstrate the utility of better time resolution in basic research in the excitotoxic model of neuronal data and the pharmacology of the dopaminergic and serotonergic systems; 4) extending the application field to different compound groups (amino acids and carbohydrates) and different markets (the Japanese clinical market for homovanillic acid and vanilyl mandelic acid assay in neonate neuroblastoma screening); and 5) initiating a theory and application publication sequence to lay the groundwork for market penetration in Phase III.