TECHNOLOGY FOR DNA DAMAGE MARKERS IN CERVICAL CANCER

原型碳柱切换(CCS)系统(1)已用于测量PAP涂片和宫颈阴道灌洗液样本中DNA加合物的游离水平，表明可能与各种危险因素和癌症有关。初步工作已经确定了这种测量在早期诊断或治疗监测中的一些可能的作用。拟议的工作旨在完善、推广和整合相关标记的识别和常规测量技术。所提出的方法是一项技术/方法论增强和标记意义识别之间的迭代研究，从大约400例病例中确定，涉及广泛的抽样方式、风险因素和诊断。 建议的商业应用： 这项拟议的工作旨在开发将DNA损伤与宫颈癌风险联系起来的研究和临床仪器。商业市场是生物医学研究、治疗监测和临床筛查。

Prototype carbon column switching (CCS) systems (1) have been applied to measuring free levels of DNA adducts in PAP smear and cervico vaginal lavage sample indicating a possible correlation with various risk factors and cancer. Preliminary work has defined some of the possible role of utility of such measurements in early diagnosis or therapy monitoring. The proposed work is directed at refining, extending and integrating the technology for the identification and routine measurement of relevant markers. The proposed approach is an iterative study between technology/methodology enchancements and marker significance identification from a cohort of ca. 400 cases spanning a wide range of sampling modalities, risk factors and diagnoses. PROPOSED COMMERCIAL APPLICATION: The proposed work is directed at developing research and clinical instrumentation for relating DNA damage to cercical cancer risk. The commercial markets are in biomedical research, therapy monitoring, and clinical screening.