Expression cloning of the gene that transform Caco-2 cells to M cells
Expression cloning of the gene that transform Caco-2 cells to M cells
批准号:
22790343
负责人:
KOBAYASHI Motohiro
金额:
$2.5万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2011
中文摘要
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英文摘要
Our research goal was to clone the gene that transforms Caco-2 cells into M cells, which transcytose antigenic molecules in the intestinal lumen to subepithelial immune cells, by employing the Caco-2/Raji cells co-culture system. While we tried to find optimal condition for the induction of M cell phenotype, reproducible condition was not identified. Moreover, the transfection efficiency of Caco-2 cells by a lipofection method was too low to perform expression cloning.
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Production of an antibody against long-form N-acetylglucosamine-6-O-sulfotransferase 1 that is expressed in human high endothelial venules
生产针对人高内皮小静脉中表达的长型 N-乙酰葡糖胺-6-O-磺基转移酶 1 的抗体
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Fujiwara M, et al]
通讯作者:
et al
Deficiency of L-selectin ligand biosynthesis by knocking out of GlcNAc6ST-1 and-2 suppresses asthmatic reponse in murine model of asthma
通过敲除 GlcNAc6ST-1 和-2 导致 L-选择素配体生物合成缺陷可抑制小鼠哮喘模型中的哮喘反应
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[Bao X, Nakayama J, Miler M, Pham A, Kobayashi M, Kawashima H, Broide D, Fukuda M]
通讯作者:
Fukuda M
Induction of high endothelial venule-like vessels expressing GlcNAc6ST-1-mediated L-selectin ligand carbohydrate and mucosal addressin cell adhesion molecule 1(MAdCAM-1) in a mouse model of" Candidatus Helicobacter heilmannii"-induced gastritis and gastri
在“海尔曼螺杆菌”诱导的胃炎和胃炎小鼠模型中诱导表达 GlcNAc6ST-1 介导的 L-选择素配体碳水化合物和粘膜寻址素细胞粘附分子 1(MAdCAM-1)的高内皮小静脉样血管
DOI:
10.1111/j.1523-5378.2010.00801.x
发表时间:
2010
期刊:
Helicobacter
影响因子:
4.4
作者:
[Suzuki A, Kobayashi M, Matsuda K, Matsumoto T, Kawakubo M, Kumazawa S, Koide N, Miyagawa S, Ota H]
通讯作者:
Ota H
Induction of HEV-like vessels expressing GlcNAc6ST-1-mediated L-selectin ligand carbohydrate and MAdCAM-1 in a mouse model of "Candidatus Helicobacter heilmannii"-induced gastritis and MALT lymphoma
在“海尔曼螺杆菌”诱导的胃炎和 MALT 淋巴瘤小鼠模型中诱导表达 GlcNAc6ST-1 介导的 L-选择素配体碳水化合物和 MAdCAM-1 的 HEV 样血管
DOI:
--
发表时间:
2010
期刊:
Helicobacter
影响因子:
4.4
作者:
[Suzuki A, et al]
通讯作者:
et al
ο-glycans carrying non-sulfated sialyl Lewis X appearing on HEV-like vessels in gastric MALT lymphoma function as L-selectin ligands
胃 MALT 淋巴瘤中 HEV 样血管中出现的携带非硫酸化唾液酸 Lewis X 的 ο-聚糖作为 L-选择素配体
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[Kobayashi M, Mitoma J, Hoshino H, Yu SY, Shimojo Y, Suzawa K, Khoo KH, Fukuda M, Nakayama J]
通讯作者:
Nakayama J
共 11 条
Histological analysis of ulcerative colitis using human pathology samples and mice deficient in sulfotransferases
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批准号:15K08343
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$3.08万
-
财政年份:2015
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负责人:KOBAYASHI Motohiro
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依托单位:
Expression cloning of the gene functioning in transformation of Caco-2 cells to M cells
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批准号:24590410
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.41万
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财政年份:2012
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负责人:KOBAYASHI Motohiro
-
依托单位:
Basic Research on Political Part of China towards Tokyo Trial
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批准号:23520880
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.41万
-
财政年份:2011
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负责人:KOBAYASHI Motohiro
-
依托单位:
Analysis of sulfotransferases involved in biosynthesis of selectin ligand carbohydrates in ulcerative colitis
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批准号:20790278
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项目类别:Grant-in-Aid for Young Scientists (B)
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资助金额:$2.66万
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财政年份:2008
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负责人:KOBAYASHI Motohiro
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依托单位:
海外基金