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Expression cloning of the gene that transform Caco-2 cells to M cells

Expression cloning of the gene that transform Caco-2 cells to M cells
Caco-2细胞向M细胞转化基因的表达克隆
批准号:
22790343
负责人:
KOBAYASHI Motohiro
金额:
$2.5万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2011

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中文摘要
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英文摘要
Our research goal was to clone the gene that transforms Caco-2 cells into M cells, which transcytose antigenic molecules in the intestinal lumen to subepithelial immune cells, by employing the Caco-2/Raji cells co-culture system. While we tried to find optimal condition for the induction of M cell phenotype, reproducible condition was not identified. Moreover, the transfection efficiency of Caco-2 cells by a lipofection method was too low to perform expression cloning.
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Production of an antibody against long-form N-acetylglucosamine-6-O-sulfotransferase 1 that is expressed in human high endothelial venules
生产针对人高内皮小静脉中表达的长型 N-乙酰葡糖胺-6-O-磺基转移酶 1 的抗体
DOI: --
发表时间: 2011
期刊:
影响因子: --
作者: [Fujiwara M, et al]
通讯作者: et al
Deficiency of L-selectin ligand biosynthesis by knocking out of GlcNAc6ST-1 and-2 suppresses asthmatic reponse in murine model of asthma
通过敲除 GlcNAc6ST-1 和-2 导致 L-选择素配体生物合成缺陷可抑制小鼠哮喘模型中的哮喘反应
DOI: --
发表时间: 2010
期刊:
影响因子: --
作者: [Bao X, Nakayama J, Miler M, Pham A, Kobayashi M, Kawashima H, Broide D, Fukuda M]
通讯作者: Fukuda M
Induction of high endothelial venule-like vessels expressing GlcNAc6ST-1-mediated L-selectin ligand carbohydrate and mucosal addressin cell adhesion molecule 1(MAdCAM-1) in a mouse model of" Candidatus Helicobacter heilmannii"-induced gastritis and gastri
在“海尔曼螺杆菌”诱导的胃炎和胃炎小鼠模型中诱导表达 GlcNAc6ST-1 介导的 L-选择素配体碳水化合物和粘膜寻址素细胞粘附分子 1(MAdCAM-1)的高内皮小静脉样血管
DOI: 10.1111/j.1523-5378.2010.00801.x
发表时间: 2010
期刊: Helicobacter
影响因子: 4.4
作者: [Suzuki A, Kobayashi M, Matsuda K, Matsumoto T, Kawakubo M, Kumazawa S, Koide N, Miyagawa S, Ota H]
通讯作者: Ota H
Induction of HEV-like vessels expressing GlcNAc6ST-1-mediated L-selectin ligand carbohydrate and MAdCAM-1 in a mouse model of "Candidatus Helicobacter heilmannii"-induced gastritis and MALT lymphoma
在“海尔曼螺杆菌”诱导的胃炎和 MALT 淋巴瘤小鼠模型中诱导表达 GlcNAc6ST-1 介导的 L-选择素配体碳水化合物和 MAdCAM-1 的 HEV 样血管
DOI: --
发表时间: 2010
期刊: Helicobacter
影响因子: 4.4
作者: [Suzuki A, et al]
通讯作者: et al
11
    Histological analysis of ulcerative colitis using human pathology samples and mice deficient in sulfotransferases
    • 批准号:
      15K08343
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.08万
    • 财政年份:
      2015
    • 负责人:
      KOBAYASHI Motohiro
    • 依托单位:
    Expression cloning of the gene functioning in transformation of Caco-2 cells to M cells
    Basic Research on Political Part of China towards Tokyo Trial
    Analysis of sulfotransferases involved in biosynthesis of selectin ligand carbohydrates in ulcerative colitis
    • 批准号:
      20790278
    • 项目类别:
      Grant-in-Aid for Young Scientists (B)
    • 资助金额:
      $2.66万
    • 财政年份:
      2008
    • 负责人:
      KOBAYASHI Motohiro
    • 依托单位:
    海外基金