TOXIC CONGENER SPECIFIC IMMUNOASSAY FOR PCBS

PCBS 有毒同源物特异性免疫测定

• 批准号: 2390801
• 负责人: ROBERT E CARLSON
• 金额: $ 20.97万
• 依托单位: ECOCHEM RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-30 至 1998-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2390801
• 关键词: chemical structure function; chemical synthesis; environmental toxicology; halobiphenyl /halotriphenyl compound; haptens; hybridomas; immunoaffinity chromatography; immunologic assay /test; isomer; laboratory mouse; method development; monoclonal antibody

Polychlorinated biphenyls (PCBs) are ubiquitous environmental pollutants with diverse toxic, teratogenic, reproductive, immunotoxic and tumorigenic effects. Three of the least abundant chlorinated PCB isomers (congeners) are the most toxic and most difficult to quantify. Novel competitors, a novel hapten and a monoclonal antibody (MAb) were prepared in Phase I and utilized to create a sensitive and specific immunoassay for two of the three most toxic PCB congeners. This Phase II project expands on the Phase I goals to produce new chemical competitors and MAbs which will recognize the remaining highly toxic congener and two groups of slightly less toxic but much more abundant congeners. Chemometric methods will be used with the immunoassays to identify the major classes of PCB congeners in environmental and biological samples. MAb immunoaffinity reagents will be prepared for selective recovery and chromatography of toxic congeners from crude samples, and for development of advanced field-portable and automated instrumental immunoassay formats. This project combines the research and development expertise of ECOCHEM, the University of California, Berkeley Hybridoma Facility and the product manufacturing, field evaluation and validation experience of the EnviroGard project team at Millipore Corp. This effort will yield the first commercial assays and reagents for congener-specific PCB identification and quantitation in environmental and biological samples. PROPOSED COMMERCIAL APPLICATION: Commercial applications include products for regulatory hazard assessment, occupational exposure monitoring, bioremediation, environmental toxicology research, environmental remediation, fish and wildlife protection and food safety monitoring.

多氯联苯是一种普遍存在的环境污染物。 具有多种毒性、致畸性、生殖性、免疫毒性和 致癌作用。三种最不丰富的氯化多氯联苯异构体 (同系物)是毒性最大、最难量化的。小说 竞争对手，制备了一种新的半抗原和一种单抗 在第一阶段，并用于建立灵敏和特异的免疫分析 三种毒性最强的多氯联苯中的两种。本项目二期工程 在第一阶段目标的基础上扩展以生产新的化学品竞争对手和单抗 它将识别剩余的剧毒同系物和两组 毒性稍小但含量更丰富的同系物。化学计量学 方法将与免疫分析一起使用，以确定主要类别 环境和生物样品中的多氯联苯同系物。单抗 将制备免疫亲和试剂，用于选择性回收和 用于开发的粗制样品中有毒同系物的层析 先进的现场便携和自动化仪器免疫分析 格式。该项目结合了以下项目的研发专业知识 加州大学伯克利分校杂交院和 的产品制造、现场评估和验证经验 米利波尔公司的EnviroGard项目团队这一努力将产生 首个用于同类产品特定印刷电路板的商业化分析和试剂 环境和生物样品中的鉴定和定量。 建议的商业应用：商业应用包括 监管危害评估、职业暴露用产品 监测、生物修复、环境毒理学研究、 环境修复、鱼类和野生动物保护以及食品安全 监控。