TRICHLOROETHYLENE BAIT-AND-SWITCH IMMUNOASSAY

描述（由申请人提供）：卤化溶剂，包括氯乙烯、三氯乙烯、1，2-二氯乙烷和氯仿，是超级基金场地的重要污染源。 其中最重要的卤化溶剂是三氯乙烯（TCE）。 三氯乙烯是一种商品溶剂;仅美国的产量就高达每年30万吨。 三氯乙烯的药理学和毒理学已被广泛研究。 三氯乙烯是一种致癌物质和肝毒素。 三氯乙烯也是一种普遍存在的环境污染物。 人类接触三氯乙烯的主要来源是在工作场所以及通过受污染的土壤、沉积物和地下水，特别是危险废物场地附近。 目前可用的TCE测定方法通常基于气相色谱分析。 这些方法需要专用仪器，并且不容易适应现场或快速响应应用。 酶免疫测定（EIA）已被反复证明在现场、高通量和/或工作现场监测的简单性、速度和成本方面比基于仪器的方法具有显著优势。 该计划的目标是开发对环境重要和超级基金关键的卤化溶剂的免疫测定。 该项目的目标是开发一种三氯乙烯诱饵-转换免疫测定法，该方法将：（1）用于危险废物场地监测，（2）作为开发卤化溶剂类环境污染物诱饵-转换策略免疫测定法的概念验证原型。

DESCRIPTION (provided by applicant): The halogenated solvents, which include vinyl chloride, trichloroethylene,1,2 dichloroethane and chloroform, are a significant source of contamination at Superfund Sites. Among the most important of the halogenated solvents is trichloroethylene (TCE). TCE is a commodity solvent; production levels in the United States alone have been as high as 300,000 tons per year. The pharmacology and toxicology of TCE have been extensively studied. TCE is a carcinogen and a hepatic toxin. TCE is also a ubiquitous environmental contaminant. The primary sources of human exposure to TCE are in the workplace and through contaminated soil, sediment and groundwater, especially near hazardous waste sites. The currently available methods for TCE determination are typically based on gas chromatographic analysis. These methods require dedicated instrumentation and are not readily adapted to field or quick response applications. Enzyme ImmunoAssay (EIA) has repeatedly been demonstrated to have significant advantages over instrument-based methods in terms of simplicity, speed and cost for field, high throughput and/or worksite monitoring. The goal of this program is the development of immunoassays for the environmentally-important and Superfund-critical halogenated solvents. The goal of this project is the development of a TCE bait-and-switch immunoassay which will: (1) be used for hazardous waste site monitoring, and (2) serve as the proof-of-concept prototype for the development of bait-and-switch strategy immunoassays for the halogenated solvent class of environmental contaminants.