TOXIC CONGENER-SPECIFIC IMMUNOASSAY FOR PCB'S

多氯联苯（PCBs）在海洋中的广泛存在， 环境需要全面评估其环境 冲击 由于以下事实，这一评估变得更加困难 多氯联苯是209种可能的氯异构体的混合物， 其中常见的可能不代表最重要的毒性或其他 生物活性成分。 因此， 认识到， 多氯联苯的环境评价需要环境 冲击 然而，与分析相关的工作和费用 这些化合物通过毛细管气相色谱法 对所需研究的限制。 基于免疫测定的分析方法 已经证明能够提供高通量筛选 和定量分析。 这种方法提供了一个显着减少 由于高特异性和仪器化， 由于分析简单，要求。 该项目将建立在 ECOCHEM成功开发出灵敏、定量的Aroclor 定向PCB免疫测定，以开发毒性/生物活性 用于样本筛查和同系物特异性的同系物相关检测 分析.

The widespread occurrence of polychlorinated biphenyls (PCBs) in the environment requires a comprehensive assessment of their environmental impact. This assessment is made substantially more difficult by the fact that the PCBs are a mixture of 209 possible chloro- isomers, the most common of which may not represent the most significant toxic or otherwise biologically active components. Consequently, there is growing recognition that the congener specific analysis of PCBs in the environment is required for the evaluation of the PCBs environmental impact. However, the effort and expense associated with the analysis of these compounds by capillary gas chromatography place substantial restraints on the required studies. Immunoassay based analytical methods have demonstrated the ability to provide both high throughput screening and quantitative analyses. This method provides for a marked reduction in both sample preparation due to high specificity and instrumentation requirements due to assay simplicity. This project will build on ECOCHEM's successful development of a sensitive and quantitative Aroclor directed PCB immunoassay to develop a toxic / biologically active congener relevant assay for both sample screening and congener specific analysis.