TOXIC CONGENER-SPECIFIC IMMUNOASSAY FOR PCB'S

PCB 有毒同源物特异性免疫测定

• 批准号: 3493607
• 负责人: ROBERT E CARLSON
• 金额: $ 4.98万
• 依托单位: ECOCHEM RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-30 至 1994-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3493607
• 关键词: Chordata; environmental toxicology; enzyme linked immunosorbent assay; halobiphenyl /halotriphenyl compound; isomer; mass spectrometry; method development; monoclonal antibody; nuclear magnetic resonance spectroscopy

The widespread occurrence of polychlorinated biphenyls (PCBs) in the environment requires a comprehensive assessment of their environmental impact. This assessment is made substantially more difficult by the fact that the PCBs are a mixture of 209 possible chloro- isomers, the most common of which may not represent the most significant toxic or otherwise biologically active components. Consequently, there is growing recognition that the congener specific analysis of PCBs in the environment is required for the evaluation of the PCBs environmental impact. However, the effort and expense associated with the analysis of these compounds by capillary gas chromatography place substantial restraints on the required studies. Immunoassay based analytical methods have demonstrated the ability to provide both high throughput screening and quantitative analyses. This method provides for a marked reduction in both sample preparation due to high specificity and instrumentation requirements due to assay simplicity. This project will build on ECOCHEM's successful development of a sensitive and quantitative Aroclor directed PCB immunoassay to develop a toxic / biologically active congener relevant assay for both sample screening and congener specific analysis.

多氯联苯(PCbs)在世界各地的广泛存在 环境要求对其环境进行全面评估 冲击力。由于以下事实，这一评估变得更加困难 多氯联苯是209种可能的氯异构体的混合物，最 其中常见的可能不代表最显著的毒性或其他 生物活性成分。因此，有越来越多的 认识到对环境中多氯联苯的同类专门分析 环境是多氯联苯环境评价所必需的 冲击力。然而，与分析有关的工作和费用 这些化合物经毛细管气相色谱分析后， 对所需学习的限制。基于免疫分析的分析方法 已经证明有能力提供高通量筛查 和定量分析。这种方法提供了显著的减少 在这两种样品制备中，由于高特异性和仪器 由于分析的简单性，要求。这个项目将建立在 Ecochem公司成功研制出灵敏、定量的Aroclore 定向多氯联苯免疫分析发展毒性/生物活性 用于样品筛选和同种特异性的同种物质相关分析 分析。

项目成果

A monoclonal immunoassay for the coplanar polychlorinated biphenyls.

共面多氯联苯的单克隆免疫分析。

• DOI: 10.1021/ac00117a003
• 发表时间: 1995
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Chiu,YW;Carlson,RE;Marcus,KL;Karu,AE
• 通讯作者: Karu,AE