Mechanobiology: a new model of integrin activation by membrane tension

力学生物学：膜张力激活整合素的新模型

• 批准号: DE190100609
• 负责人: A/Prof Lining (Arnold) Ju
• 金额: $ 25.84万
• 依托单位: The University of Sydney
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Early Career Researcher Award
• 财政年份: 2019
• 资助国家: 澳大利亚
• 起止时间: 2019-07-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DE190100609
• 关键词: Mechanobiology; new; model; integrin; activation

This project aims to address a fundamental question in mechanobiology on how integrin receptor coordinates with force to mediate cell spreading, migration, growth and survival. With an observation that membrane deformation enhances integrin binding, the project expects to establish a new model of integrin activation by membrane tension. It will develop an integrated approach combining single-molecule force probes, super resolution microscopy, microfluidics and molecular dynamics simulations. It is expected that the role of membrane tension in promoting cell adhesion will be defined at molecular scale, and the link between integrin activation and Piezo calcium channel mediated membrane tension sensing will be delineated.

本项目旨在解决机械生物学中的一个基本问题，即整合素受体如何与力协调，介导细胞的扩散、迁移、生长和存活。通过观察膜变形增强整合素结合，该项目期望建立膜张力激活整合素的新模型。它将开发一种结合单分子力探针、超分辨率显微镜、微流体和分子动力学模拟的综合方法。预期膜张力在促进细胞粘附中的作用将在分子水平上被定义，并且整合素活化和压电钙通道介导的膜张力传感之间的联系将被描绘。