CHROMOSOME SPECEFIC DNA PROBES FOR LABORATORY SPECIES

荧光染色体特异的DNA探针允许识别 间期细胞和有丝分裂细胞染色体异常的多样性 包括非整倍体和断裂/重排事件，开辟了一个新的领域 被称为“分子细胞遗传学”的遗传学研究的一部分。整体 染色体探针和染色体区域特异性探针可用于 人类和小鼠的染色体，但很少有这样的探针可用于大鼠 用于繁殖的其他实验室物种的染色体， 发育和癌症研究。我们建议为下列人士提供这类调查 大鼠、兔和非人类灵长类动物的染色体DNA分析 显微解剖及细菌人工材料的制作和筛选 染色体(BAC)文库。具体来说，在第二阶段，我们将开发 大鼠、兔和恒河猴的染色体特异点探针， 以及兔子和恒河猴部分子集的全染色体涂抹探针 染色体。兔、兔全染色体涂布探针的选择 恒河猴将通过与有经验的合作者协商确定 体内遗传毒理学和生殖生物学。此外，我们 建议开发一套用于大鼠和人类的探针，用于 染色体鉴定，无需Giemsa显带或其他 更难的染色程序。

Fluorescent chromosome-specific DNA probes allow identification of a variety of chromosome abnormalities in interphase and mitotic cells including aneuploids and breakage/rearrangement events, opening a new area of genetic investigation known as "molecular cytogenetics". Whole chromosome probes and chromosome region-specific probes are available for human and mouse chromosomes, but few such probes are available for rat chromosomes of other laboratory species used in reproductive, developmental, and cancer studies. We propose to provide such probes for rat, rabbit and non-human primate chromosomes by chromosomal DNA microdissection and by making and screening bacterial artificial chromosome (BAC) libraries. Specifically, in Phase II we will develop chromosome-specific point probes for the rat, rabbit, and rhesus macaque, and whole chromosome paint probes for a subset of rabbit and rhesus chromosomes. The choice of whole chromosome paint probes for rabbit and rhesus will be determined by consultation with collaborators experienced in in vivo genetic toxicology and reproductive biology. In addition, we propose to develop sets of probes for rat and human which will be used for chromosome identification, without the need for Giemsa banding or other more difficult staining procedures.