MOUSE SUB-CHROMOSOMAL DNA PAINT PROBES

我们建议开发一套完整的亚染色体荧光， 小鼠基因组的原位杂交（FISH）探针。探测器将在 基于染色体显微切割和含有大的 小鼠基因组插入物。基于显微解剖的探针将用于 光谱显带，多色单染色体FISH技术， 分析所有类型的亚染色体重排因为光谱 显带探针组（每条染色体最多7个探针）将被键入到 标准Giemsa带型，用户将能够描述他们的 常规G-显带染色体检查的结果。基于YAC的集 的连锁细胞遗传学FISH探针间隔约5厘米的间隔，将 开发了一个细胞遗传学带锚定连锁图， 老鼠.我们还建议建立一套光谱显带探针， 人类核型，并使用小鼠连锁细胞遗传学探针， 协调人/小鼠共映射。这种物种间的协调 细胞遗传学图谱，当与跨物种FISH，将允许 研究人员利用其他哺乳动物物种来接触丰富的人类 和小鼠基因数据库，从而扩大了这些的商业市场， probes.

We propose to develop a complete set of sub-chromosomal fluorescence in situ hybridization (FISH) probes for the mouse genome. The probes will be based on both chromosomal microdissection and YAC clones containing large mouse genomic inserts. The microdissection-based probes will be used for spectral banding, a multi-color single chromosome FISH technique to analyze all types of sub-chromosomal rearrangements. Because the spectral banding probe sets (up to 7 probes per chromosome) will be keyed to the standard Giemsa banding pattern, users will be able to describe their findings in terms of conventional G-banding momenclature. A YAC-based set of linkage-cytogenetic FISH probes spaced at about 5 cM intervals, will be developed to coordinate a cytogenetic band-anchored linkage map for the mouse. We also propose to build a set of spectral banding probes for the human karyotype and to use the mouse linkage-cytogenetic probes to coordinate human/mouse co-maps. This interspecies coordination of cytogenetic maps, when used with cross-species FISH, will allow researchers using other mammalian species to have access to the rich human and mouse gene databases, thus widening the commercial market for these probes.