MOUSE SUB-CHROMOSOMAL DNA PAINT PROBES

我们建议开发一套完整的亚染色体荧光 小鼠基因组的原位杂交(FISH)探针。探测器将是 基于染色体显微切割和包含大片段的YAC克隆 小鼠基因组插入。基于显微解剖的探针将用于 光谱显带，一种多色单染色体FISH技术 分析所有类型的亚染色体重排。因为光谱 显带探针组(每个染色体最多7个探针组)将与 标准Giemsa条带模式，用户将能够描述他们的 在传统的G-显带时刻标记方面的发现。基于YAC的集合 间隔约5厘米的连锁-细胞遗传学FISH探针，将 为协调细胞遗传学带锚定的连锁图谱而开发 老鼠。我们还建议建立一套光谱带状探测器，用于 人的核型，并使用小鼠连锁细胞遗传学探针 协调人/鼠的联合贴图。这种物种间的协调 细胞遗传学图谱，当用于跨物种鱼类时，将允许 研究人员利用其他哺乳动物物种接触到富有的人类 和老鼠基因数据库，从而扩大了这些产品的商业市场 探测器。

We propose to develop a complete set of sub-chromosomal fluorescence in situ hybridization (FISH) probes for the mouse genome. The probes will be based on both chromosomal microdissection and YAC clones containing large mouse genomic inserts. The microdissection-based probes will be used for spectral banding, a multi-color single chromosome FISH technique to analyze all types of sub-chromosomal rearrangements. Because the spectral banding probe sets (up to 7 probes per chromosome) will be keyed to the standard Giemsa banding pattern, users will be able to describe their findings in terms of conventional G-banding momenclature. A YAC-based set of linkage-cytogenetic FISH probes spaced at about 5 cM intervals, will be developed to coordinate a cytogenetic band-anchored linkage map for the mouse. We also propose to build a set of spectral banding probes for the human karyotype and to use the mouse linkage-cytogenetic probes to coordinate human/mouse co-maps. This interspecies coordination of cytogenetic maps, when used with cross-species FISH, will allow researchers using other mammalian species to have access to the rich human and mouse gene databases, thus widening the commercial market for these probes.